Team:Heidelberg/pages/Collaborations

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Revision as of 00:19, 18 October 2014


Besides spending a whole summer in the lab and developing an own project, iGEM is about meeting new people, people with the same interests, the same motivation, the same dedication. Collaborations between team ensure the exchange of ideas, therefore helping the field of Synthetic Biology to be interdisciplinary and creative - the best conditions to give rise to revolutionary projects. We are very happy to met some of the team members from Freiburg, Aachen, Tuebingen, Marburg and London.

Contents

iGEM Team Aachen

Michael from the iGEM team Aachen visited us in Heidelberg. Their team worked on the development of a real-time pathogen detection technique for what they have build their own fluorescence measurement camera. We provided our expression vectors to the iGEM team Aachen, which they used to express their part E1010-K1319003, the human galectin-3 protein fused to mRFP. Nicely seen in Figure 1 and 2 the the intensity of the red color is significantly higher for the induced sample with pSBX1A30 compared to the uninduced ones. To ensure the right molecular mass of the fusion protein, a SDS gel was run as well (Figure 3).

3) SDS-PAGE of K1319020 expression
3) SDS-PAGE of K1319020 expression

2) Expression of Galectin-3
2) Expression of Galectin-3

1) Expression of Galectin-3
1) Expression of Galectin-3

More information about their project can be found on Aachens Galectin-3 site.

In exchange they offered to characterise these expression vectors with their own designed analyser by measuring the amount of produced fluorescent proteins. Unfortunately, transporting E.colis to another city wasn't as easy as expected, not all Bacteria were able to grow afterwards and we could only obtain sufficient data from pSBX4C5.


iGEM Team Tuebingen

The project of iGEM Team Tuebingen is about blood types and the difficulties that arises during blood donation of the wrong blood type. They aim to develop an easy system to convert blood types A, B, AB to the rares but also most applicable blood type 0. They have applied the method of intein targeting, a method which can also be found in our intein toolbox, to the N-Acetyl-Galactosaminidase protein. More about their project can be read in the very nice article they have wrote for our tumblr blog or on their own wiki.

In this collaboration, Tuebingen conducted a mass spectrometry analysis of our Lambda lysozyme to proof circularization. The data still has to be evaluated, but we will have them ready until the jamboree. There is a high demand for our expression vectors. Tuebingen had problems with expressing their constructs, therefore we send them some of our plasmids pSBX1K30 (BBa_K1362093) and pSBX4K50 (BBa_K1362097), as they showed to work really nicely even with big proteins like the Dnmt1 with 105kDa.


iGEM Team Freiburg

Three of our team members, Anna, Caro, and Charlotte visited the iGEM team Freiburg. We met the team right after a big team meeting, therefore we were able to hear their latest project developments. We spend the evening talking about experiences in the lab and about iGEM. It was relieving to hear that they faced similar difficulties in the lab. The morning after we presented our project to the iGEM team Freiburg. Freiburg was very interested in our program iGEM@home as we offered them to promote their own human practice projects. You can also find an article about optogenetics on our tumblr blog. Both our teams are working with light inducible systems, manly the LOV domain. Although working in different organisms, the constructs were compatible, therefore we could interchange protocols for induction and preliminary results. Furthermore we gazed at their professional light induction box - we can definitely improved ours.

After cooperation with the iGEM Team Freiburg for two years in a row we are hoping to keep Freiburg as a reliable partner in the next years as well.


iGEM Team Marburg

The screensaver of iGEM@home is a great possibility to spread news and information about Synthetic Biology around the world. Lately, even other iGEM team have used this new option and filled our screensaver with slides about their own project. Marburg was able to promote their quiz app about Synthetic Biology.