http://2014.igem.org/wiki/index.php?title=Team:Heidelberg/Project/Reconstitution&feed=atom&action=history
Team:Heidelberg/Project/Reconstitution - Revision history
2024-03-28T21:21:22Z
Revision history for this page on the wiki
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http://2014.igem.org/wiki/index.php?title=Team:Heidelberg/Project/Reconstitution&diff=338124&oldid=prev
Pirwlan at 17:54, 17 October 2014
2014-10-17T17:54:20Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>abstract= <p><del class="diffchange diffchange-inline">Placeholder</del></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>abstract= <p><ins class="diffchange diffchange-inline">In our intein toolbox we provide fusion and activation as principle adaptable for nearly all proteins that exhibit a modular structure. To prove this principle we split superfold GFP in two halves at amino acid position 64/65 and joined each to the corresponding split intein NpuDnaE. In this experiment we successfully reassemble the protein halves and restored fluorescence of sfGFP. The fluorescence was measured via TECAN plate reader and FACS analysis, as well as visualized on the Western Blot. Split fluorescent proteins provide an easy to use read-out for the efficiency of intein splicing and can be further used for large screenings as utilized in our LOV-mutant screen </ins></p></div></td></tr>
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Pirwlan
http://2014.igem.org/wiki/index.php?title=Team:Heidelberg/Project/Reconstitution&diff=310796&oldid=prev
Pirwlan at 08:57, 17 October 2014
2014-10-17T08:57:38Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>title=<del class="diffchange diffchange-inline">Reconstituion </del>of Fluorescence</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>title=<ins class="diffchange diffchange-inline">Restoration </ins>of Fluorescence</div></td></tr>
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Pirwlan
http://2014.igem.org/wiki/index.php?title=Team:Heidelberg/Project/Reconstitution&diff=310749&oldid=prev
Pirwlan at 08:56, 17 October 2014
2014-10-17T08:56:31Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>title=Reconstituion of <del class="diffchange diffchange-inline">fluorescent Proteins</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>title=Reconstituion of <ins class="diffchange diffchange-inline">Fluorescence</ins></div></td></tr>
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Pirwlan
http://2014.igem.org/wiki/index.php?title=Team:Heidelberg/Project/Reconstitution&diff=310730&oldid=prev
Pirwlan at 08:56, 17 October 2014
2014-10-17T08:56:04Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>title=<del class="diffchange diffchange-inline">FUSING PROTEINS</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>title=<ins class="diffchange diffchange-inline">Reconstituion of fluorescent Proteins</ins></div></td></tr>
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Pirwlan
http://2014.igem.org/wiki/index.php?title=Team:Heidelberg/Project/Reconstitution&diff=310702&oldid=prev
Bunnech at 08:55, 17 October 2014
2014-10-17T08:55:23Z
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Bunnech
http://2014.igem.org/wiki/index.php?title=Team:Heidelberg/Project/Reconstitution&diff=310689&oldid=prev
Bunnech at 08:55, 17 October 2014
2014-10-17T08:55:03Z
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Bunnech
http://2014.igem.org/wiki/index.php?title=Team:Heidelberg/Project/Reconstitution&diff=310656&oldid=prev
Bunnech at 08:54, 17 October 2014
2014-10-17T08:54:06Z
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Bunnech
http://2014.igem.org/wiki/index.php?title=Team:Heidelberg/Project/Reconstitution&diff=308045&oldid=prev
Pirwlan: Created page with "{{:Team:Heidelberg/templates/wikipage_new| title=Bringing parts together | white=true | red-logo=true | header-img= | header=background-color:#DE4230 | header-bg=black | subtitl..."
2014-10-17T07:37:38Z
<p>Created page with "{{:Team:Heidelberg/templates/wikipage_new| title=Bringing parts together | white=true | red-logo=true | header-img= | header=background-color:#DE4230 | header-bg=black | subtitl..."</p>
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Pirwlan