Team:Heidelberg

From 2014.igem.org

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        <h3 class="normal-medium-text">iGEM TEAM <span>HEIDELBERG</span> 2014</h3>
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<h3 class="normal-medium-text">iGEM TEAM <span>HEIDELBERG</span> 2014</h3>
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        <h1 class="very-large-text">THE RING<br />OF <span>FIRE</span></h1>
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<h1 class="very-large-text">THE RING<br />OF <span>FIRE</span></h1>
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<p> Click <a href="/Team:Heidelberg/Project#Abstract">here</a> to view our abstract.
<p> Click <a href="/Team:Heidelberg/Project#Abstract">here</a> to view our abstract.
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Scroll down to EXPLORE our project.
Scroll down to EXPLORE our project.
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        <p><span class="larger red-text">Nature</span> has made many curious inventions. One of these are</p>
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<p><span class="larger red-text">Nature</span> has made many curious inventions. One of these are</p>
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        <h1 class="very-large-text red-text bold" style="text-align:right;">CIRCULAR PROTEINS</h1>
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<h1 class="very-large-text red-text bold" style="text-align:right;">CIRCULAR PROTEINS</h1>
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        <p>which are unconventional peptides that neither have a beginning, nor an ending</p>
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<p>which are unconventional peptides that neither have a beginning, nor an ending</p><br>
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        <p class="normal-medium-text bold">These proteins are extremely <span class="red-text">resistant</span> against
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<p class="normal-medium-text bold">These proteins are extremely <span class="red-text">resistant</span> against
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  <span class="red-text">high temperatures</span>, <span class="red-text">pH</span> and <span class="red-text">proteases</span>.</p>
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  <span class="red-text">high temperatures</span>, pH and proteases.</p>
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        <p class="normal-medium-text align-right">We established protein circularization as a <span class="red-text">new<br/>powerful tool</span> for Synthetic Biology and set the foundations<br />to render any protein heat stable.</p>
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<p class="normal-medium-text align-right">We established protein circularization as a new<br>powerful tool for Synthetic Biology and set the foundations<br>to render any protein heat stable.</p>
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<h1 class="large-text bold">Wondering how we circularize?</h1>
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      <h2 class="medium-text align-right">Let us introduce you to the next generation of bioengineering...</h2>
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<h2 class="normal-medium-text align-right bold">Let us introduce you to the next generation of bioengineering...</h2>
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      <h2 class="medium-text align-right">COME DISCOVER THE MECHANISM OF SPLIT INTEINS</h2>
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<h2 class="align-right" style="font-size: 40px;">come DISCOVER the MECHANISM of SPLIT INTEINS</h2><br><br>
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        <p>Inteins excise themselves out of proteins and in doing so the remaining flanking parts are irreversibly joined</p>
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        <p>&ndash;&nbsp;an effective mechanism to circularize proteins.</p>
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<p>Inteins excise themselves out of proteins and in doing so the remaining flanking parts are irreversibly joined</p>
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<p>–&nbsp;an effective mechanism to circularize proteins.</p>
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          <p class="normal-medium-text">But messing with protein structure can be disastrous,<br/><span class="medium-text">you can only win with good MODELING.</span></p>
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          <p><span class="larger">Find out the EXCITING THEORY<br>behind RIGID LINKERS and their ANGLES</span></p>
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<p class="normal-medium-text align-right">But messing with protein structure can be disastrous,<br><span class="medium-text">you can only win with good MODELING.</span></p><br><br>
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<p class="normal-medium-text"><span>Find out the <span class="bold">EXCITING THEORY</span><br>behind <span class="bold">RIGID LINKERS</span> and their <span class="bold">ANGLES</span></span></p>
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          <p class="normal-medium-text">We developed CRAUT,<br/>
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          a comprehensive software which identifies<br />
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          the <span class="red-text">optimal path</span> to <span class="red-text">connect</span> a protein&rsquo;s <span class="red-text">termini</span><br/>
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a comprehensive software which identifies<br>
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          preserving structure and function.</p>
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preserving structure and function.</span></p>
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          <p><span class="larger">Look at our extensive wet-lab SCREENING to improve and calibrate our software using <span class="red-text">lambda lysozyme</span></span></p>
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<p><span class="larger">Look at our extensive wet-lab SCREENING to improve and calibrate our software using <a class="red-text" href="/Team:Heidelberg/Project/Linker_Screening">lambda lysozyme</a>!</span></p>
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            <p class="align-right"><span class="larger">As calculations on protein structures are costly<br />we involved the rest of the world with</span></p>
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            <h1 class="very-large-text red-text"><span style="font-size:1.5em;">iGEM@home</span></h1>
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<p class="align-right"><span class="larger">As calculations on protein structures are costly<br>we involved the rest of the world with</span></p>
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"><span style="font-size:1.5em;">iGEM@home</span></h1>
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        <h3 class="normal-medium-text">Empowering science!</h3>
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        <p>NEW PLATFORM for distributed computing.</p>
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        <p>Volunteers provide the idle calacity of their home computers.</p>
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        <p>And, with an established user base with more than <span class="larger red-text">1000</span> computers, we effectively bring synthetic biology to society in a new way</p>
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<h3 class="medium-text bold">Empowering science!</h3><br>
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<p class="normal-medium-text">NEW PLATFORM for distributed computing.</p>
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<p><span class="larger">Volunteers provide the idle calacity of their home computers.</span></p>
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<p class=""><span class="larger">And, with an established user base with more than <span class="larger dark-red-text">1000</span> computers, we effectively bring synthetic biology to society in a new way</span></p>
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        <p>Wouldn´t it be great to amplify DNA in a normal PCR maintaining the epigenetic information coded in methylation patterns?</p>
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<h1 class="dark-grey-text" style="text-align: right;"><span style="font-size: 0.8em;">circular <span class="red-text">heat-stable</span></span><br>DNMT1</h1>
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<p>Wouldn´t it be great to amplify DNA in a normal PCR maintaining the epigenetic information coded in methylation patterns?</p>
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            <img src="/wiki/images/5/58/Heidelberg_Toolbox_Circularization.png" id="circ-icon" class="toolbox-icon">&nbsp;&nbsp;CIRCULARIZATION
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<img src="/wiki/images/5/58/Heidelberg_Toolbox_Circularization.png" id="circ-icon" class="toolbox-icon">&nbsp;&nbsp;CIRCULARIZATION
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        <h1 style="text-align: right; font-size: 3em;" >... and show you the  WORLD of <br />
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        <p>The iGEM Team Heidelberg has developed an intein toolbox for the iGEM community to easily modify your protein in a standarized method.</p>
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<h1 style="text-align: right; font-size: 3em;" >... and show you the  WORLD of <br />
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        <p>Our toolbox contains several tools which are PLACEHOLDER. Here you can find out more about our Toolbox.</p>
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<span class="red-text">post-translational MODIFCATION</span>
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        <p>In addition to that all tools are inducible by light. Using the LOV system we built a solid method for regulation of the intein trans-splicing reaction our toolbox consiting on. Click here to get more informations about Induction.</p>
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<p>In addition to that all tools are inducible by light. Using the LOV system we built a solid method for regulation of the intein trans-splicing reaction our toolbox consiting on. Click here to get more informations about Induction.</p>
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          <h1 style="text-align: right; color: black; padding-right: 130px;" ><span style="font-size: 0.8em;">circular <span style="color:white;">heat-stable</span></span><br><span style="font-size:1.3em;">Xylanase</span></h1>
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          <p>Xylanase is an important enzyme for the pulp and paper industry.</p>
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          <p>Bla bla</p>
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          <p>In future Xylanase could be used for the production of biofuel.</p>
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<p>Xylanase is an important enzyme for the pulp and paper industry.</p>
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<p>In future Xylanase could be used for the production of biofuel.</p>
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          <span class="title-dnmt1">INDUSTRY</span>
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        <h1>Who are we?</h1>
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        <p>We are the iGEM Team Heidelberg 2014 consisting of 12 highly motivated bachelor and master students studying at Heidelberg University.</p>
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        <p>For our project we got great feedback and support from our supervisors.</p>
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        <p>Take a look at our <a href="/Team:Heidelberg/Team">Teampage</a>!</p>
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<p>We are the iGEM Team Heidelberg 2014 consisting of 12 highly motivated bachelor and master students studying at Heidelberg University.</p>
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        <p>We want thank all people who helped us and supported our work in the lab.</p>
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<p>For our project we got great feedback and support from our supervisors.</p>
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<p>Take a look at our <a href="/Team:Heidelberg/Team">Teampage</a>!</p>
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Revision as of 15:30, 15 October 2014

iGEM Logo
Ring of fire Image

iGEM TEAM HEIDELBERG 2014

THE RING
OF FIRE

Click here to view our abstract.
Scroll down to EXPLORE our project.

Nature has made many curious inventions. One of these are

CIRCULAR PROTEINS

which are unconventional peptides that neither have a beginning, nor an ending


These proteins are extremely resistant against high temperatures, pH and proteases.

We established protein circularization as a new
powerful tool for Synthetic Biology and set the foundations
to render any protein heat stable.

Wondering how we circularize?

Let us introduce you to the next generation of bioengineering...

come DISCOVER the MECHANISM of SPLIT INTEINS



Inteins excise themselves out of proteins and in doing so the remaining flanking parts are irreversibly joined

– an effective mechanism to circularize proteins.

Placeholder

But messing with protein structure can be disastrous,
you can only win with good MODELING.



Find out the EXCITING THEORY
behind RIGID LINKERS and their ANGLES


We developed CRAUT,
a comprehensive software which identifies
the optimal path to connect a protein’s termini
preserving structure and function.

Look at our extensive wet-lab SCREENING to improve and calibrate our software using lambda lysozyme!

As calculations on protein structures are costly
we involved the rest of the world with

iGEM@home

Empowering science!


NEW PLATFORM for distributed computing.

Volunteers provide the idle calacity of their home computers.

And, with an established user base with more than 1000 computers, we effectively bring synthetic biology to society in a new way

circular heat-stable
DNMT1

Wouldn´t it be great to amplify DNA in a normal PCR maintaining the epigenetic information coded in methylation patterns?

The problem: DNMT 1, an enzyme which is responsible for the establishment and maintenance of the individual methylation pattern of different cell types, is not heat stable. For iGEM 2014 we therefore create a PCR 2.0 with heat-stable DNMT 1 by circularization.

PCR 2.0

... and show you the WORLD of
post-translational MODIFCATION

The iGEM Team Heidelberg has developed an intein toolbox for the iGEM community to easily modify your protein in a standarized method.

Our toolbox contains several tools which are PLACEHOLDER. Here you can find out more about our Toolbox.

In addition to that all tools are inducible by light. Using the LOV system we built a solid method for regulation of the intein trans-splicing reaction our toolbox consiting on. Click here to get more informations about Induction.

circular heat-stable
Xylanase

Xylanase is an important enzyme for the pulp and paper industry.

Bla bla

In future Xylanase could be used for the production of biofuel.

INDUSTRY

Who are we?

We are the iGEM Team Heidelberg 2014 consisting of 12 highly motivated bachelor and master students studying at Heidelberg University.

For our project we got great feedback and support from our supervisors.

Take a look at our Teampage!

Thank you!

We want thank all people who helped us and supported our work in the lab.

Retrieved from "http://2014.igem.org/Team:Heidelberg"