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Team:Heidelberg
From 2014.igem.org
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<div class="col-lg-6 col-md-6 col-sm-12 impressiv"> | <div class="col-lg-6 col-md-6 col-sm-12 impressiv"> | ||
<div class="hidden-sm hidden-xs" style="padding-top:100px;"></div> | <div class="hidden-sm hidden-xs" style="padding-top:100px;"></div> | ||
| - | <h3 class="normal- | + | <h3 class="normal-medium-text">iGEM TEAM <span>HEIDELBERG</span> 2014</h3> |
<h1 class="very-large-text">THE RING<br />OF <span>FIRE</span></h1> | <h1 class="very-large-text">THE RING<br />OF <span>FIRE</span></h1> | ||
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<h1 class="very-large-text red-text bold" style="text-align:right;">CIRCULAR PROTEINS</h1> | <h1 class="very-large-text red-text bold" style="text-align:right;">CIRCULAR PROTEINS</h1> | ||
<p>which are unconventional peptides that neither have a beginning, nor an ending</p> | <p>which are unconventional peptides that neither have a beginning, nor an ending</p> | ||
| - | <p class="normal- | + | <p class="normal-medium-text bold">These proteins are extremely <span class="red-text">resistant</span> against |
<span class="red-text">high temperatures</span>, <span class="red-text">pH</span> and <span class="red-text">proteases</span>.</p> | <span class="red-text">high temperatures</span>, <span class="red-text">pH</span> and <span class="red-text">proteases</span>.</p> | ||
| - | <p class="normal- | + | <p class="normal-medium-text align-right">We established protein circularization as a <span class="red-text">new<br/>powerful tool</span> for Synthetic Biology and set the foundations<br />to render any protein heat stable.</p> |
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<div class="jumbotron slide red red-ring"> | <div class="jumbotron slide red red-ring"> | ||
<h1 class="large-text">Wondering how we circularize?</h1> | <h1 class="large-text">Wondering how we circularize?</h1> | ||
| - | <h2 class=" | + | <h2 class="medium-text align-right">Let us introduce you to the next generation of bioengineering...</h2> |
| - | <h2 class=" | + | <h2 class="medium-text align-right">COME DISCOVER THE MECHANISM OF SPLIT INTEINS</h2> |
<div class="col-lg-6"> | <div class="col-lg-6"> | ||
<p>Inteins excise themselves out of proteins and in doing so the remaining flanking parts are irreversibly joined</p> | <p>Inteins excise themselves out of proteins and in doing so the remaining flanking parts are irreversibly joined</p> | ||
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<p class="align-right "></p> | <p class="align-right "></p> | ||
| - | <p class="normal- | + | <p class="normal-medium-text">But messing with protein structure can be disastrous,<br/><span class="medium-text">you can only win with good MODELING.</span></p> |
<p>Find out the EXCITING THEORY<br>behind RIGID LINKERS and their ANGLES</p> | <p>Find out the EXCITING THEORY<br>behind RIGID LINKERS and their ANGLES</p> | ||
<br/> | <br/> | ||
| - | <p class="normal- | + | <p class="normal-medium-text">We developed CRAUT,<br/> |
a comprehensive software which identifies<br /> | a comprehensive software which identifies<br /> | ||
the <span class="red-text">optimal path</span> to <span class="red-text">connect</span> a protein’s <span class="red-text">termini</span><br/> | the <span class="red-text">optimal path</span> to <span class="red-text">connect</span> a protein’s <span class="red-text">termini</span><br/> | ||
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<div class="col-md-9 col-xs-12"> | <div class="col-md-9 col-xs-12"> | ||
| - | < | + | <p class="larger align-right">As calculations on protein structures are costly<br />we involved the rest of the world with</p> |
| + | <h1 class="very-large-text red-text">iGEM@home</h1> | ||
</div> | </div> | ||
<div class="clearfix"></div> | <div class="clearfix"></div> | ||
</div> | </div> | ||
<div class="col-md-8 col-xs-12"> | <div class="col-md-8 col-xs-12"> | ||
| + | <h3 class="normal-medium-text">Empowering science!</h3> | ||
<p><br/>After calculating for eleven days and the breakdown of both computational and mental power we decided to spread the modeling of the linkers.</p> | <p><br/>After calculating for eleven days and the breakdown of both computational and mental power we decided to spread the modeling of the linkers.</p> | ||
<p>The iGEM Team Heidelberg developed <a href="/Team:Heidelberg/Software/igemathome">iGEM@home</a>, a software to divide extensive computing task into many packages and to distribute them to many computers. Now over 1,000 volunteers are calculating for us when their computers are idle.</p> | <p>The iGEM Team Heidelberg developed <a href="/Team:Heidelberg/Software/igemathome">iGEM@home</a>, a software to divide extensive computing task into many packages and to distribute them to many computers. Now over 1,000 volunteers are calculating for us when their computers are idle.</p> | ||
Revision as of 14:18, 15 October 2014
iGEM TEAM HEIDELBERG 2014
THE RING
OF FIRE
Click here to view our abstract.
Scroll down to EXPLORE our project.
CIRCULARIZATION
OLIGOMERIZATION
FUSION
ON/OFF
PURIFICATION
... and show you the WORLD of
post-translational MODIFCATION
The iGEM Team Heidelberg has developed an intein toolbox for the iGEM community to easily modify your protein in a standarized method.
Our toolbox contains several tools which are PLACEHOLDER. Here you can find out more about our Toolbox.
In addition to that all tools are inducible by light. Using the LOV system we built a solid method for regulation of the intein trans-splicing reaction our toolbox consiting on. Click here to get more informations about Induction.
circular heat-stable
DNMT1
Wouldn´t it be great to amplify DNA in a normal PCR maintaining the epigenetic information coded in methylation patterns?
The problem: DNMT 1, an enzyme which is responsible for the establishment and maintenance of the individual methylation pattern of different cell types, is not heat stable. For iGEM 2014 we therefore create a PCR 2.0 with heat-stable DNMT 1 by circularization.
circular heat-stable
Xylanase
Xylanase is an important enzyme for the pulp and paper industry.
Bla bla
In future Xylanase could be used for the production of biofuel.
As calculations on protein structures are costly
we involved the rest of the world with
iGEM@home
Empowering science!
After calculating for eleven days and the breakdown of both computational and mental power we decided to spread the modeling of the linkers.
The iGEM Team Heidelberg developed iGEM@home, a software to divide extensive computing task into many packages and to distribute them to many computers. Now over 1,000 volunteers are calculating for us when their computers are idle.
As a new tool for the iGEM community this system enables every student team to archieve their modeling without access to big server farms.
Who are we?
We are the iGEM Team Heidelberg 2014 consisting of 12 highly motivated bachelor and master students studying at Heidelberg University.
For our project we got great feedback and support from our supervisors.
Take a look at our Teampage!
Thank you!
We want thank all people who helped us and supported our work in the lab.
