"
Colony PCR on more transformants containing pSB1C3 with NisB and NisRK
was repeated, using VF2 and
VR. This time, a
correct insert size wasbfound for a NisRK transformant.
Because there was still no correct insert size found for a NisB
transformant, the remaining NisB colonies were tested for a correct insert size.
There were no positive transformants found.
