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Team:Groningen/Template/MODULE/Notebook/bandage/week10
From 2014.igem.org
Figure 2:
Fig. 1: our L.lactis bacteria, seen through a phase contrast microscope (every white dot is GFP)
8 September - 12 September
Redone the diffusion experiments. This time after I put the acrylamid gel on the agar plate I've also put some liquid M17 agar around the edges to make sure everything will touch the agar. This was put overnight in the 30 °C stove. We were hoping for some nice halo's around the acrylamid gels.
As you can see on the picture this experiment worked as expected. Nisin's ability to create no growth area's are undoubtedly proven with these experiments. We've also tried this with putting the acrylamid circles for two hours in media or water. We expected that our cells would give a larger halo when grown with media. This result was different, there was no real visible difference between those two.
