Revision as of 22:16, 16 October 2014

Wet lab

Our experimental approach can be summarized as follows:

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Number	Gene	Organism	pIGA (pDONR+GOI in E.coli)	pIGB (pDEST+GOI for AH109)	AIG (derived from AH109)	Y2H (interaction partners)
1	mp65	N/A	X	X	X	X
2	tos1	N/A	X	X	X	X
3	sim1	N/A	X	X	X	X
4	ssr1	N/A	X	X	X	X
5	pir4	N/A	X	X	X	X
6	scw4	N/A	X	X	X	X
7	pir3	N/A	X	X	N/A	N/A
8	utr2	N/A	X	X	N/A	N/A
9	crf2full	A. fumigatus	X	X	X	X
10	crf2act	A. fumigatus	X	X	X	X
11	bglEno	A. fumigatus	X	N/A	N/A	N/A
12	ecm33	A. fumigatus	X	X	X	X
13	sun1	A. fumigatus	X	X	X	X
14	als1	N/A	X	X	X	X
15	als3	N/A	X	X	X	X
16	rodA	A. fumigatus	X	X	N/A	N/A
17	rodA	A. nidulans	X	X	X	X
18	xlnA	A. nidulans	X	X	X	X
19	npc2	A. nidulans	X	X	X	X
20	sho1	A. nidulans	X	X	X	X
21	MID2	S. cerevisiae	X	X	X	X
22	SED1	S. cerevisiae	X	X	X	X
23	prm1 B	A. fumigatus	X	X	N/A	N/A
24	crf1	A. fumigatus	X	X	X	X
25	ama1	T. gondii	X	X	X	X
26	eglC	A. fumigatus	X	N/A	N/A	N/A
27	CWP1	S. cerevisiae	X	X	N/A	N/A
28	CWP2	S. cerevisiae	X	X	N/A	N/A
29	TIR4	S. cerevisiae	X	N/A	N/A	N/A
30	rom5	T. gondii	X	N/A	N/A	N/A
31	rom4	T. gondii	X	N/A	N/A	N/A
32	gra7	T. gondii	X	N/A	N/A	N/A
33	eglC	A. nidulans	X	N/A	N/A	N/A
34	TIR1	S. cerevisiae	X	X	N/A	N/A
35	bglEex	A. fumigatus	X	X	N/A	N/A

Number	Gene	Organism	pIGZ (p1170 from diploid cells for E.coli)	pIGY (p1170 from E.coli for Y187)	YIG (derived from Y187)	PepTag
1	mp65		X	X	X
2	tos1		X	X	X
3	sim1		X	X	X
4	ssr1		X	X	X	X
5	pir4		X	X	X	X
6	scw4		X	X	X
7	pir3		N/A	N/A	N/A
8	utr2		N/A	N/A	N/A
9	crf2full	A. fumigatus	X	X	X
10	crf2act	A. fumigatus	X	X	X
11	bglEno	A. fumigatus	N/A	N/A	N/A
12	ecm33	A. fumigatus	X	X	X
13	sun1	A. fumigatus	X	X	X	X
14	als1		X	X	X
15	als3		X	X	X
16	rodA	A. fumigatus	N/A	N/A	N/A
17	rodA	A. nidulans	X	X	X
18	xlnA	A. nidulans	N/A	N/A	N/A
19	npc2	A. nidulans	X	X	X
20	sho1	A. nidulans	N/A	N/A	N/A
21	MID2	S. cerevisiae	X	X	X
22	SED1	S. cerevisiae	N/A	N/A	N/A
23	prm1 B	A. fumigatus	N/A	N/A	N/A
24	crf1	A. fumigatus	X	X	X
25	ama1	T. gondii	X	X	X
26	eglC	A. fumigatus	N/A	N/A	N/A
27	CWP1	S. cerevisiae	N/A	N/A	N/A
28	CWP2	S. cerevisiae	N/A	N/A	N/A
29	TIR4	S. cerevisiae	N/A	N/A	N/A
30	rom5	T. gondii	N/A	N/A	N/A
31	rom4	T. gondii	N/A	N/A	N/A
32	gra7	T. gondii	N/A	N/A	N/A
33	eglC	A. nidulans	N/A	N/A	N/A
34	TIR1	S. cerevisiae	N/A	N/A	N/A
35	bglEex	A. fumigatus	N/A	N/A	N/A

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 <h1>Wet lab</h1>