"
Team:Goettingen/proLP
From 2014.igem.org
(Difference between revisions)
Gwen Eleven (Talk | contribs) m |
Gwen Eleven (Talk | contribs) m |
||
| Line 52: | Line 52: | ||
<li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Bradford">Bradford Assay</a></li> | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_protein#Bradford">Bradford Assay</a></li> | ||
</ul> | </ul> | ||
| - | <h4><a href="https://2014.igem.org/Team:Goettingen/ | + | <h4><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo"><i>In vivo</i> tests</a></li> |
<ul> | <ul> | ||
| - | <li><a href="https://2014.igem.org/Team:Goettingen/ | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#Prod">Production of spore suspension</a></li> |
| - | <li><a href="https://2014.igem.org/Team:Goettingen/ | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#Culti_A">Cell cultivation</a></li> |
| - | <li><a href="https://2014.igem.org/Team:Goettingen/ | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#immu">Immunofluorescence Staining </a></li> |
| - | <li><a href="https://2014.igem.org/Team:Goettingen/ | + | <li><a href="https://2014.igem.org/Team:Goettingen/protocol_inVivo#Calc">Calcofluor staining</a></li> |
| - | + | ||
| - | + | ||
</ul> | </ul> | ||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 12:51, 17 October 2014
Overview
PCR Methods
Plasmid Construction
- Restriction of DNA
- Ligation of DNA fragments
- BP recombination reaction
- LR recombination reaction
- SEAMLESS Cloning
- Peptide Library construction
Plasmid Transformation
- E.coli competent cells
- Plasmid isolation (E.coli)
- E.coil transformation
- Plasmid isolation (Yeast)
- Yeast transformation
