Team:Evry/Overview/Achievements/Projects

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Projects Achievements

Chassis characterization

- Genome Sequencing of Pseudovibrio denitrificans to collect informations defense mechanisms,
transposons putative insertion localisation and to possess a assembled genome for RNAseq.

- Growth conditions optimization

- Antibiotic sensitivity tests shown a potential resistance to tetracycline and ampicilline

- Electroporation Transformation protocol establishment that never been done before

New Vector

- Transposon-based vector was created to overcome resistance mechanisms to DNA entrance

- Proof of concept to use it in Pseudovibrio denitrificans was achieved

New or Optimized sensors Sensors

- New PCB sensor based on bphR1 promoter and binding protein bphR2.

- An optimized version of the Phenol sensors from Pekin iGEM 2013 team was constructed

- RNA-seq data shall be used to find new promoters

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