Team:Evry/Notebook/Transposons/09.18.14

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Picture

Golden Gate Is10

A PCR with primer Golden Gate Is10, which amplified the part between Is10 and iS10 in pNK2 is realized. But this time the Tm is modified : Tm= 60°C instead of 55°C.
After migration of product PCR, there haven't bands.

Sep 18