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Team:Evry/Notebook/Sensing/Phenol/09-18-2014
From 2014.igem.org
(Difference between revisions)
| Line 13: | Line 13: | ||
Ligation of all parts <br> | Ligation of all parts <br> | ||
Transformation on <i>E.coli</i> <br> | Transformation on <i>E.coli</i> <br> | ||
| - | Purification of palsmids with | + | Purification of palsmids with NucleoSpin Plamsid Kit (Macherey Nagel) on 4 colonies <br> |
Digestion with EcoRI <br> | Digestion with EcoRI <br> | ||
| - | <img src="https://static.igem.org/mediawiki/2014/ | + | <img src="https://static.igem.org/mediawiki/2014/0/0d/18annot%C3%A9.jpg"/> <br> |
<br> | <br> | ||
Revision as of 03:13, 18 October 2014
Assembling of the parts
Ligation of all parts
Transformation on E.coli
Purification of palsmids with NucleoSpin Plamsid Kit (Macherey Nagel) on 4 colonies
Digestion with EcoRI
DIGEST E GFP32DMPR C1-C2-C3-PARENT C4
