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- | <h4>Week 7
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- | <h4>Interlab Study
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- | <h2> 08.12.2014 </h2>
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- | <br/> The 4 needed parts are: BBa_J23101, BBa_J23115, BBa_E0240 and BBa_I20260. Corresponding wells were located on 2014 Distribution kit plates and resuspended with 10 µL steril water. That permits to obtain a DNA concentration around 0.2 ng/µl (according to the registry. Solutions were transferred into 1 ml eppendorf tubes and stored at -20°C.
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- | <br/> To amplify fragments, a PCR was performed on the 4 constructions.
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- | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/a/a8/PCR12082014.jpg" width="500px;" class="thumbimage"/>
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- | <a href="https://static.igem.org/mediawiki/2014/a/a8/PCR12082014.jpg" class="internal" title="Enlarge">
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- | <center>Table 1: PCR mix preparation </center>
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- | <br/> Distribution of 49 µl of mix per PCR tube. Application of program IGEM Q5 PCR.
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- | <center>Table 1: IGEM Q5 PCR program thermocycling conditions </center>
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- | Preparation of a 1% agarose gel: 0.56 g of Top Vision agarose (Thermo Scientific) + 50 ml of TAE 1X (marque)
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- | Microwave 30s by 30s until agarose total dissolution
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- | gel cooling down, adding of one '"goutte" of BET
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- | Loading gel with 10µl per sample added with 1µl of loading dye (ladder Purple 2-Log ladder NEB
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- | Gel running 45 minutes at 100 mV
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- | <h2> 08.13.2014 </h2>
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- | <h2> 08.14.2014 </h2>
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