Team:Evry/Notebook/Interlabstudy/Week7

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<br/> The 4 needed parts are: BBa_J23101, BBa_J23115, BBa_E0240 and BBa_I20260. Corresponding wells were located on 2014 Distribution kit plates and resuspended with 10 µL steril water. That permits to obtain a DNA concentration around 0.2 ng/µl (according to the registry. Solutions were transferred into 1 ml eppendorf tubes and stored at -20°C.
<br/> The 4 needed parts are: BBa_J23101, BBa_J23115, BBa_E0240 and BBa_I20260. Corresponding wells were located on 2014 Distribution kit plates and resuspended with 10 µL steril water. That permits to obtain a DNA concentration around 0.2 ng/µl (according to the registry. Solutions were transferred into 1 ml eppendorf tubes and stored at -20°C.
<br/> To amplify fragments, a PCR was performed on the 4 constructions.
<br/> To amplify fragments, a PCR was performed on the 4 constructions.
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    <center>Table 1: PCR mix preparation </center>
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<br/> Distribution of 49 µl of mix per PCR tube. Application of program IGEM Q5 PCR.
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     <center>Table 1: PCR mix preparation </center>
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     <center>Table 1: IGEM Q5 PCR program thermocycling conditions </center>
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Preparation of a 1% agarose gel: 0.56 g of Top Vision agarose (Thermo Scientific) + 50 ml of TAE 1X (marque)
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Microwave 30s by 30s until agarose total dissolution
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gel cooling down, adding of one '"goutte" of BET
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Loading gel with 10µl per sample added with 1µl of loading dye (ladder Purple 2-Log ladder NEB
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Gel running 45 minutes at 100 mV
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Revision as of 12:41, 15 August 2014

Week 7

Interlab Study

08.12.2014


The 4 needed parts are: BBa_J23101, BBa_J23115, BBa_E0240 and BBa_I20260. Corresponding wells were located on 2014 Distribution kit plates and resuspended with 10 µL steril water. That permits to obtain a DNA concentration around 0.2 ng/µl (according to the registry. Solutions were transferred into 1 ml eppendorf tubes and stored at -20°C.
To amplify fragments, a PCR was performed on the 4 constructions.
IMAGE
Symbol
Table 1: PCR mix preparation

Distribution of 49 µl of mix per PCR tube. Application of program IGEM Q5 PCR.
IMAGE
Symbol
Table 1: IGEM Q5 PCR program thermocycling conditions
Preparation of a 1% agarose gel: 0.56 g of Top Vision agarose (Thermo Scientific) + 50 ml of TAE 1X (marque) Microwave 30s by 30s until agarose total dissolution gel cooling down, adding of one '"goutte" of BET Loading gel with 10µl per sample added with 1µl of loading dye (ladder Purple 2-Log ladder NEB Gel running 45 minutes at 100 mV

08.13.2014

08.14.2014

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