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Team:Evry/Notebook/Interlabstudy/Week7
From 2014.igem.org
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<br/> The 4 needed parts are: BBa_J23101, BBa_J23115, BBa_E0240 and BBa_I20260. Corresponding wells were located on 2014 Distribution kit plates and resuspended with 10 µL steril water. That permits to obtain a DNA concentration around 0.2 ng/µl (according to the registry. Solutions were transferred into 1 ml eppendorf tubes and stored at -20°C. | <br/> The 4 needed parts are: BBa_J23101, BBa_J23115, BBa_E0240 and BBa_I20260. Corresponding wells were located on 2014 Distribution kit plates and resuspended with 10 µL steril water. That permits to obtain a DNA concentration around 0.2 ng/µl (according to the registry. Solutions were transferred into 1 ml eppendorf tubes and stored at -20°C. | ||
<br/> To amplify fragments, a PCR was performed on the 4 constructions. | <br/> To amplify fragments, a PCR was performed on the 4 constructions. | ||
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| + | <div class="center"> | ||
| + | <div class="thumb tnone"> | ||
| + | <div class="thumbinner" style="width:502px;"> | ||
| + | <a href="URL IMAGE" class="image"> | ||
| + | <img alt="IMAGE" src="URL IMAGE" width="500px;" class="thumbimage"/> | ||
| + | </a> | ||
| + | <div class="thumbcaption"> | ||
| + | <div class="magnify"> | ||
| + | <a href="URL IMAGE" class="internal" title="Enlarge"> | ||
| + | <img src="https://2014.igem.org/File:PCR12082014.jpg" width="15" height="11" alt="Symbol"/> | ||
| + | </a> | ||
| + | </div> | ||
| + | <center>Table 1: PCR mix preparation </center> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
Revision as of 12:34, 15 August 2014
Week 7
Interlab Study
08.12.2014
The 4 needed parts are: BBa_J23101, BBa_J23115, BBa_E0240 and BBa_I20260. Corresponding wells were located on 2014 Distribution kit plates and resuspended with 10 µL steril water. That permits to obtain a DNA concentration around 0.2 ng/µl (according to the registry. Solutions were transferred into 1 ml eppendorf tubes and stored at -20°C.
To amplify fragments, a PCR was performed on the 4 constructions.
Table 1: PCR mix preparation
08.13.2014
08.14.2014
Interlab Study
08.12.2014
The 4 needed parts are: BBa_J23101, BBa_J23115, BBa_E0240 and BBa_I20260. Corresponding wells were located on 2014 Distribution kit plates and resuspended with 10 µL steril water. That permits to obtain a DNA concentration around 0.2 ng/µl (according to the registry. Solutions were transferred into 1 ml eppendorf tubes and stored at -20°C.
To amplify fragments, a PCR was performed on the 4 constructions.
