Team:Evry/Notebook

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<li><a  href="https://2014.igem.org/Team:Evry/Notebook">Notebook</a>
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<h1>IGEM Evry <span id="typeout-text"></span> 2014</h1>
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<!--*************************************** WEEK XX ****************************************************************-->
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<h1 id="week2">Week 2: 28<sup>th</sup> July - 3<sup>th</sup> August</h1>
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<h2>Friday, 1th August</h2>
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<b><div align="center">Protocol for transformation </b><br/></div>
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We transformed TOP 10 E. coli with plasmid of conjugation. This plasmid contains erythromycin like resistance cassette. I plated them on LB-Agar with erythomycin antibiotic.
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<h2>Sunday, 3st August</h2>
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<b><div align="center">Transformed cells </b><br/></div>
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The cells transformed groth in the plate with erythromycin. I take one colonie in 3mL LB with erythromycin.
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<b><div align="center">Tests culture pseudovibrio with medium </b><br/></div>
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I take 1mL culture in M9 (aa and 3% NaCL)to 3 differents medium :<br/>
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9mL MB<br/>
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9mL M9<br/>
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9mL 2xYT<br/>
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9mL LB<br/>
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<h1 id="week1">Week 1: 4<sup>th</sup>August - 10<sup>rd</sup> August</h1>
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<h2>Monday, 4th August</h2>
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<b><div align="center">Tests culture pseudovibrio with medium </b><br/></div>
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MB : the cells growth<br/>
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M9 : the cells growth<br/>
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2xYT : the cells growth<br/>
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LB : the cells don't growth<br/>
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<b><div align="center">Plasmid DNA puriication </b><br/></div>
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A culture of E.coli transformed with plasmide of conjugation. A plasmid purification is realised with kit NucleoSpin.
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After that 94,5ng.L-1 is purified.
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<h2>Tuesday, 5th August</h2>
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A culture of DH5a pir transformed with pNK2 (plasmid use to the transposas) in LB with ampicillin growth.
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After that the plasmid s purified.
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Revision as of 14:02, 12 August 2014

IGEM Evry 2014

IGEM Evry 2014

Biology
Conference

Biology project

Modeling
Workshops

Model's

Policy and Practices
Hackathons

Policy and Practices

17
Team members
2
Biobricks
3
Collaborations
239+
All-nighters


Spon-ge-sors

Donators

Nicolas Cornille, Yves Mansoulie, Kevin Berenger, Benoît Monjanel, Pierre Parutto, Laurence Catatao, Hélène Gontier, Fatouma Sanou, Paul Besançon, Adrien Isidore, Diane Thomas, Odile Boyer, Julie Zaworski, Bernard Jesop, Jean-Louis Leuci, Hélène Dumant, Maurice Gontier, Aurélie Bérenger, Sylvie Currias, Maximin Rousseill, Florian Duval, Bastien Maysseng, Samuel Parmentier, Muriel Walter, Xavier Ferbeck, Béatrice Besançon, Alain Bauer, Gérard Vilarem.

Retrieved from "http://2014.igem.org/Team:Evry/Notebook"