"
Team:Evry/Interlab Study/09-18-2014
From 2014.igem.org
(Difference between revisions)
| (12 intermediate revisions not shown) | |||
| Line 20: | Line 20: | ||
<br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2> <br> | <br/><h2> Construction n°3: PSB1C3 with K823012-E1010</h2> <br> | ||
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).<br> | Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).<br> | ||
| - | <img src="" alt="PLAN DE PLAQUE" /> | + | <img src="https://static.igem.org/mediawiki/2014/4/49/TECANnumber3.jpg" alt="PLAN DE PLAQUE" /> |
<br> | <br> | ||
| - | |||
| - | + | ||
| + | |||
<h2>Other constructions of the Anderson library of constitutive promoters</h2> | <h2>Other constructions of the Anderson library of constitutive promoters</h2> | ||
<br/> | <br/> | ||
<i>PSB1C3+E0240+J23101</i>: <br> | <i>PSB1C3+E0240+J23101</i>: <br> | ||
| - | + | <li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. | |
| - | <li>Pre-culture | + | <li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h43-23h43).<br> |
| - | <li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 ( | + | <img src="https://static.igem.org/mediawiki/2014/6/65/PlaqueLeft.png" alt="PLAN DE PLAQUE" /> |
| - | < | + | |
| - | <img src="" alt="PLAN DE PLAQUE" /> | + | |
<br> | <br> | ||
<br> | <br> | ||
<i>PSB1C3+E0240+J23102</i>: <br> | <i>PSB1C3+E0240+J23102</i>: <br> | ||
| - | Pre-culture | + | <li>Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. |
| + | <li>Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h30-23h30). <br> | ||
| + | <img src="https://static.igem.org/mediawiki/2014/6/62/J23114.26.09.png" alt="PLAN DE PLAQUE" /> | ||
<br> | <br> | ||
<br> | <br> | ||
<i>PSB1C3+E0240+J23104</i>: <br> | <i>PSB1C3+E0240+J23104</i>: <br> | ||
| - | Pre-culture | + | Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. |
<br> | <br> | ||
<br> | <br> | ||
| + | <i>PSB1C3+E0240+J23105</i>: <br> | ||
| + | glycerol stock preparation from LB culture of the 17th September. | ||
| + | <br> | ||
| + | <br> | ||
<i>PSB1C3+E0240+J23107</i>: <br> | <i>PSB1C3+E0240+J23107</i>: <br> | ||
| - | glycerol stock preparation from LB culture | + | glycerol stock preparation from LB culture of the 17th September. |
<br> | <br> | ||
<br> | <br> | ||
| Line 60: | Line 64: | ||
<i>PSB1C3+E0240+J23115</i>: <br> | <i>PSB1C3+E0240+J23115</i>: <br> | ||
| - | Pre-culture | + | Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. |
<br> | <br> | ||
<br> | <br> | ||
<i>PSB1C3+E0240+J23116</i>: <br> | <i>PSB1C3+E0240+J23116</i>: <br> | ||
| - | Pre-culture | + | Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. |
<br> | <br> | ||
<br> | <br> | ||
<i>PSB1C3+E0240+J23117</i>: <br> | <i>PSB1C3+E0240+J23117</i>: <br> | ||
| - | Pre-culture | + | Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. |
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <i>PSB1C3+E0240+J23114</i>: | ||
| + | |||
| + | |||
| + | <ul> | ||
| + | <li> PCR colony of 5 colonies for each construction following protocol table 1 and 2. | ||
| + | |||
| + | |||
| + | <div class="center"> | ||
| + | <div class="thumb tnone"> | ||
| + | <div class="thumbinner" style="width:502px;"> | ||
| + | <a href="https://static.igem.org/mediawiki/2014/7/75/114gel.jpg" class="image"> | ||
| + | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/7/75/114gel.jpg" width="450px;" class="thumbimage"/> | ||
| + | </a> | ||
| + | <div class="thumbcaption"> | ||
| + | <div class="magnify"> | ||
| + | <a href="https://static.igem.org/mediawiki/2014/7/75/114gel.jpg" class="internal" title="Enlarge"> | ||
| + | <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/> | ||
| + | </a> | ||
| + | </div> | ||
| + | <center>GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23114 construction. </center> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | </ul> | ||
| + | |||
| + | <br> | ||
| + | A pre-culture was made with one colony to make a glycerol stock.<br> | ||
<br> | <br> | ||
Latest revision as of 18:59, 17 October 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23101:
PSB1C3+E0240+J23102:
PSB1C3+E0240+J23104:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23105:
glycerol stock preparation from LB culture of the 17th September.
PSB1C3+E0240+J23107:
glycerol stock preparation from LB culture of the 17th September.
PSB1C3+E0240+J23110:
Pre-culture does'nt grow. The TECAN measure is reported.
PSB1C3+E0240+J23115:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23116:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23117:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23114:
- PCR colony of 5 colonies for each construction following protocol table 1 and 2.
GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23114 construction.
A pre-culture was made with one colony to make a glycerol stock.
Sep 18
