"
Team:Evry/Interlab Study/09-18-2014
From 2014.igem.org
(Difference between revisions)
| Line 75: | Line 75: | ||
<i>PSB1C3+E0240+J23117</i>: <br> | <i>PSB1C3+E0240+J23117</i>: <br> | ||
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. | Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C. | ||
| + | <br> | ||
<br> | <br> | ||
| - | <i>PSB1C3+E0240+ | + | |
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | <i>PSB1C3+E0240+J23115</i> and <i>PSB1C3+E0240+J23116</i>: | ||
<ul> | <ul> | ||
<li> PCR colony of 5 colonies for each construction following protocol table 1 and 2. | <li> PCR colony of 5 colonies for each construction following protocol table 1 and 2. | ||
| + | |||
<div class="center"> | <div class="center"> | ||
<div class="thumb tnone"> | <div class="thumb tnone"> | ||
<div class="thumbinner" style="width:502px;"> | <div class="thumbinner" style="width:502px;"> | ||
| - | <a href="https://static.igem.org/mediawiki/2014/ | + | <a href="https://static.igem.org/mediawiki/2014/7/75/114gel.jpg" class="image"> |
| - | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/ | + | <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/7/75/114gel.jpg" width="450px;" class="thumbimage"/> |
</a> | </a> | ||
<div class="thumbcaption"> | <div class="thumbcaption"> | ||
<div class="magnify"> | <div class="magnify"> | ||
| - | <a href="https://static.igem.org/mediawiki/2014/ | + | <a href="https://static.igem.org/mediawiki/2014/7/75/114gel.jpg" class="internal" title="Enlarge"> |
<img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/> | <img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="Symbol"/> | ||
</a> | </a> | ||
</div> | </div> | ||
| - | <center>GEL | + | <center>GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23114 construction. </center> |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| + | </ul> | ||
| - | |||
Revision as of 23:00, 16 October 2014
Construction n°1: PSB1C3 with I20260
Construction n°2: PSB1C3 with J23101-E1010
Construction n°3: PSB1C3 with K823012-E1010
Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).
Other constructions of the Anderson library of constitutive promoters
PSB1C3+E0240+J23101:
PSB1C3+E0240+J23102:
PSB1C3+E0240+J23104:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23105:
glycerol stock preparation from LB culture of the 17th September.
PSB1C3+E0240+J23107:
glycerol stock preparation from LB culture of the 17th September.
PSB1C3+E0240+J23110:
Pre-culture does'nt grow. The TECAN measure is reported.
PSB1C3+E0240+J23115:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23116:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23117:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
PSB1C3+E0240+J23115 and PSB1C3+E0240+J23116:
- PCR colony of 5 colonies for each construction following protocol table 1 and 2.
GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23114 construction.
