Team:Evry/Interlab Study/09-18-2014

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Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
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<i>PSB1C3+E0240+J23114</i>:
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        <li> PCR colony of 5 colonies for each construction following protocol table 1 and 2.
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    <img alt="IMAGE" src="https://static.igem.org/mediawiki/2014/4/45/115-116.jpg" width="450px;" class="thumbimage"/>
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    <center>GEL 2: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23116 and PSB1C3+E0240+J23115 constructions.</center>
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Revision as of 23:00, 16 October 2014

Picture

Construction n°1: PSB1C3 with I20260




Construction n°2: PSB1C3 with J23101-E1010




Construction n°3: PSB1C3 with K823012-E1010


Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).
PLAN DE PLAQUE

Other constructions of the Anderson library of constitutive promoters


PSB1C3+E0240+J23101:
  • Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h43-23h43).
    PLAN DE PLAQUE

    PSB1C3+E0240+J23102:
  • Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
  • Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h30-23h30).
    PLAN DE PLAQUE

    PSB1C3+E0240+J23104:
    Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

    PSB1C3+E0240+J23105:
    glycerol stock preparation from LB culture of the 17th September.

    PSB1C3+E0240+J23107:
    glycerol stock preparation from LB culture of the 17th September.

    PSB1C3+E0240+J23110:
    Pre-culture does'nt grow. The TECAN measure is reported.

    PSB1C3+E0240+J23115:
    Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

    PSB1C3+E0240+J23116:
    Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

    PSB1C3+E0240+J23117:
    Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.
    PSB1C3+E0240+J23114:
    • PCR colony of 5 colonies for each construction following protocol table 1 and 2.
      IMAGE
      Symbol
      GEL 2: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23116 and PSB1C3+E0240+J23115 constructions.
    Sep 18

    • Retrieved from "http://2014.igem.org/Team:Evry/Interlab_Study/09-18-2014"