"
Team:Evry/Interlab Study/08-21-2014
From 2014.igem.org
(Difference between revisions)
(Created page with "<html> <div class="cd-timeline-block"> </html> {{:Team:Evry/Notebook/Biology/TopImage}} <html> <div class="cd-timeline-content"> <p align="justify"> <br/>Transformat...") |
|||
| Line 21: | Line 21: | ||
| - | <span class="cd-date">Aug 21</span> | + | <span class="cd-date">Aug 21</span> |
</div> | </div> | ||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 13:22, 23 August 2014
Transformations of registry parts (BBa_J23115 and J23101) were performed to obtain colonies with plasmid containing parts for future amplifications.
The transformation was performed on DH5 alpha ''E. coli'', as followed:
- Remove E. coli competent tubes from -80°C and keep it on ice
- Add 3 µl of template (here solubilized plasmids from the registry distribution kit) and mix gently
- Incubate 10 minutes on ice
- Perform an heat shock 30 seconds at 42°C
- Incubate 2 minutes on ice
- Add 2 ml of LB medium and incubate 60 minutes at 37°C with an agitation at 200 rpm
- Plate 200 µl of BBa_J23115 (K823012) on a chloramphenicol LB agar plate or ampiciline for BBa_J23101
- Incubate plate overnight at 37°C
