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Team:Evry/Biology/CellCharacterization/Culture
From 2014.igem.org
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With the help of the 'Molecules of defence and communication in the microbial ecosystems' team of the National museum of natural history of Paris (their web page <a href="http://mcam.mnhn.fr/MDCEM/index.htm">here</a>), we succeed to cultivate our bacteria <i>Pseudovibrio denitrificans</i> in two medium, the marine broth and the minimal medium M9 added by 3% of NaCl.<br> | With the help of the 'Molecules of defence and communication in the microbial ecosystems' team of the National museum of natural history of Paris (their web page <a href="http://mcam.mnhn.fr/MDCEM/index.htm">here</a>), we succeed to cultivate our bacteria <i>Pseudovibrio denitrificans</i> in two medium, the marine broth and the minimal medium M9 added by 3% of NaCl.<br> | ||
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Because of the opaqueness of the marine broth, it's impossible to obtain reliable data of the growth of the bacteria. Thus, the growth curve that we have made, has been made in M9+3%NaCl 1X, with the <a id="igem-link" target="_blank" href="http://www.tecan.com/platform/apps/product/index.asp?MenuID=1813&ID=1918&Menu=1&Item=21.2.10.1.1 " title="Go to iGEM site">TECAN infiniteM200</a>. <br> | Because of the opaqueness of the marine broth, it's impossible to obtain reliable data of the growth of the bacteria. Thus, the growth curve that we have made, has been made in M9+3%NaCl 1X, with the <a id="igem-link" target="_blank" href="http://www.tecan.com/platform/apps/product/index.asp?MenuID=1813&ID=1918&Menu=1&Item=21.2.10.1.1 " title="Go to iGEM site">TECAN infiniteM200</a>. <br> | ||
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| + | <b><u>Table2:</u></b> Number of CFU on plates of MB 1X and M9+3%NaCl 1X.<br> | ||
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Revision as of 13:24, 14 October 2014
Cell culture
With the help of the 'Molecules of defence and communication in the microbial ecosystems' team of the National museum of natural history of Paris (their web page here), we succeed to cultivate our bacteria Pseudovibrio denitrificans in two medium, the marine broth and the minimal medium M9 added by 3% of NaCl.
Table1: Composition of used media, marine broth and M9+3% NaCl.
Because of the opaqueness of the marine broth, it's impossible to obtain reliable data of the growth of the bacteria. Thus, the growth curve that we have made, has been made in M9+3%NaCl 1X, with the TECAN infiniteM200.
Table2: Number of CFU on plates of MB 1X and M9+3%NaCl 1X.
![Number of CFU on plates of MB 1X and M9+3%NaCl 1X]()
