Team:ETH Zurich/project/infopro

From 2014.igem.org

(Difference between revisions)
(The goal: Emergence of patterns via information processing)
(The goal: Emergence of patterns via information processing)
Line 15: Line 15:
=== The goal: Emergence of patterns via information processing===
=== The goal: Emergence of patterns via information processing===
-
We implement a cellular automaton with bacterial cells containing our logic circuitry. Each bacterial colony serves as a core, computing an XOR gate. First, a sensor device detects the input, ''N''-acyl homoserine lactones (AHL). Check the [http://2014.igem.org/Team:ETH_Zurich/project/background/biotools#Biological_Tools quorum sensing and integrases article] for more information. Then, the cell integrates this signal through a logic gate, performed by serine integrases by sensing on the protein level and acting on the DNA level. A necessary post-processing step allows then the production of a new AHL variant due to activated gene expression through the integrase. Meanwhile, green fluorescent protein (GFP) indicates the state of the colony and serves as a long-lasting visual read out. The produced AHL output-signal then propagates in a directed fashion through a [http://2014.igem.org/Team:ETH_Zurich/lab/chip millifluidic] grid to the next bacterial colony. This iterative process faces the challenges of leakiness, cross-talk, protein-level computation and exact diffusion steps. This information pathway is shown in figure 1.
+
We implement a cellular automaton with bacterial cells containing our logic circuitry. Each bacterial colony serves as a core, computing an XOR gate. First, a sensor device detects the input, ''N''-acyl homoserine lactones (AHL). Check the [http://2014.igem.org/Team:ETH_Zurich/project/background/biotools#Biological_Tools quorum sensing and integrases article] for more information. Then, the cell integrates this signal through a logic gate, performed by serine integrases by sensing on the protein level and acting on the DNA level. A necessary post-processing step allows then the production of a new AHL variant due to activated gene expression through the integrase. Meanwhile, green fluorescent protein (GFP) indicates the state of the colony and serves as a long-lasting visual read out. The produced AHL output-signal then propagates in a directed fashion through a [http://2014.igem.org/Team:ETH_Zurich/lab/chip millifluidic] grid to the next bacterial colony. This iterative process faces the challenges of [http://2014.igem.org/Team:ETH_Zurich/expresults/rr#Riboregulators leakiness], crosstalk, protein-level computation and exact [http://2014.igem.org/Team:ETH_Zurich/modeling/diffmodel diffusion] steps. This information pathway is shown in figure 1.

Revision as of 03:37, 18 October 2014

iGEM ETH Zurich 2014