Team:ETH Zurich/project/infopro

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=== The goal: Emergence of patterns via information processing===
=== The goal: Emergence of patterns via information processing===
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We implement a cellular automaton with bacterial cells containing our logic circuitry. Each bacterial colony serves as a core, computing an XOR gate. First, a sensor device detects the input, ''N''-acyl homoserine lactones (AHL). Then, the cell integrates this signal through a logic gate, performed by serine integrases by sensing on the protein level and acting on the DNA level. A necessary post-processing step allows then the production of a new AHL variant due to activated gene expression through the integrase. Meanwhile, green fluorescent protein (GFP) indicates the state of the colony and serves as a long-lasting visual read out. The produced AHL output-signal then propagates in a directed fashion through a millifluidic grid to the next bacterial colony. This iterative process faces the challenges of leakiness, cross-talk, protein-level computation and exact diffusion steps. This information pathway is shown in figure 1.
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We implement a cellular automaton with bacterial cells containing our logic circuitry. Each bacterial colony serves as a core, computing an XOR gate. First, a sensor device detects the input, ''N''-acyl homoserine lactones (AHL). Check the [http://2014.igem.org/Team:ETH_Zurich/project/background/biotools#Biological_Tools quorum sensing and integrases article] for more information. Then, the cell integrates this signal through a logic gate, performed by serine integrases by sensing on the protein level and acting on the DNA level. A necessary post-processing step allows then the production of a new AHL variant due to activated gene expression through the integrase. Meanwhile, green fluorescent protein (GFP) indicates the state of the colony and serves as a long-lasting visual read out. The produced AHL output-signal then propagates in a directed fashion through a [http://2014.igem.org/Team:ETH_Zurich/lab/chip millifluidic] grid to the next bacterial colony. This iterative process faces the challenges of leakiness, cross-talk, protein-level computation and exact diffusion steps. This information pathway is shown in figure 1.
[[File:ETH_Zurich2014_info_processing1.png|center|800px|thumb|'''Figure 1 The information pathway in the project Mosai''coli'' on a colony level.''' Each cell colony is part of a greater memory unit (left-hand side) and gets its input information from the neighboring colonies. The input is given in the form of two different AHLs, here named A and B. This information is then step-wise processed in each of the colonies: sensing - computing - producing - sending. The output molecules serve then as the input for the next colony (middle and left-hand side). This iterative process allows the information processing from the top row of the memory unit to the bottom by chemical wiring via diffusing signals and bio-computations inside of the colonies.]]
[[File:ETH_Zurich2014_info_processing1.png|center|800px|thumb|'''Figure 1 The information pathway in the project Mosai''coli'' on a colony level.''' Each cell colony is part of a greater memory unit (left-hand side) and gets its input information from the neighboring colonies. The input is given in the form of two different AHLs, here named A and B. This information is then step-wise processed in each of the colonies: sensing - computing - producing - sending. The output molecules serve then as the input for the next colony (middle and left-hand side). This iterative process allows the information processing from the top row of the memory unit to the bottom by chemical wiring via diffusing signals and bio-computations inside of the colonies.]]
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=== The steps involved: From sensing to sending===
=== The steps involved: From sensing to sending===

Revision as of 03:35, 18 October 2014

iGEM ETH Zurich 2014