Team:ETH Zurich/project/infopro

From 2014.igem.org

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=== Sensing ===
=== Sensing ===
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Diffused HSL molecules are identified via [http://2014.igem.org/Team:ETH_Zurich/expresults#Quorum_Sensing quorum sensing]. This [http://2014.igem.org/Team:ETH_Zurich/modeling/qs integration] from a continuous variable, the concentration of HSL, to a discrete variable, the activation of a promoter, is prone to errors due to leakiness and crosstalk between HSL molecules, regulatory proteins and promoters (we characterized it for [http://parts.igem.org/Part:BBa_R0062 Lux promoter (BBa_R0062)], [http://parts.igem.org/Part:BBa_R0079 Las promoter (BBa_R0079)] and [http://parts.igem.org/Part:BBa_R0071 Rhl promoter (BBa_R0071)]). To limit the risk of error propagation, we used a [http://parts.igem.org/Part:BBa_K1541000 riboregulating system].
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Diffused HSL molecules are identified via [http://2014.igem.org/Team:ETH_Zurich/expresults#Quorum_Sensing quorum sensing machinery]. The [http://2014.igem.org/Team:ETH_Zurich/modeling/qs transfer function] between concentration of sensed HSL to promoter activation is prone to errors due to leakiness and crosstalk between HSL molecules, regulatory proteins and promoters (we characterized it for [http://parts.igem.org/Part:BBa_R0062 Lux promoter (BBa_R0062)], [http://parts.igem.org/Part:BBa_R0079 Las promoter (BBa_R0079)] and [http://parts.igem.org/Part:BBa_R0071 Rhl promoter (BBa_R0071)]). To limit the risk of error propagation, we used a [http://parts.igem.org/Part:BBa_K1541000 riboregulating system].
=== Computing ===
=== Computing ===
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=== Producing ===
=== Producing ===
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The enzyme synthesizes the signaling molecule AHL.
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To propagate the computation results, the output has to be translated again into a quorum sensing molecule. Therefore a synthase is expressed to produce a new AHL signal.
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=== Memorising ===
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Alongside the AHL synthase, GFP is produced according to the XOR output. Th fluorescent protein acts both as long term memory for the colony state and as visual signal, storing and showing the progressive pattern formation.
=== Sending ===
=== Sending ===
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The produced AHL diffuses through the channels to the rows below. The speed of this directed diffusion determines the time needed from the pattern to emerge. You can find out more information about diffusion and chip design on our [http://2014.igem.org/Team:ETH_Zurich/modeling/diffmodel modeling page], our [http://2014.igem.org/Team:ETH_Zurich/expresults#Diffusion_On_Chip experimental results page] and our [http://2014.igem.org/Team:ETH_Zurich/lab/chip chip page].
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The produced AHL diffuses through the channels to the rows below, becoming the input of the following layer. The speed of this directed diffusion determines the time needed from the pattern to emerge. You can find out more information about diffusion and chip design on our [http://2014.igem.org/Team:ETH_Zurich/modeling/diffmodel modeling page], our [http://2014.igem.org/Team:ETH_Zurich/expresults#Diffusion_On_Chip experimental results page] and our [http://2014.igem.org/Team:ETH_Zurich/lab/chip chip page].
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=== Memorising ===
 
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GFP is produced according to the XOR output. It indicates the state of the well. As it is a longlasting signal, the pattern can progressively appear.
 
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then appear.
 
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Revision as of 21:22, 17 October 2014

iGEM ETH Zurich 2014