Global Information Processing Pathway

Initating The Signal

We initiate the signal by pipetting one sort of 3OC6-HSL in a well of the first line.

Sensing

Diffused HSL molecules are identified via quorum sensing. This integration from a continuous variable, the concentration of HSL, to a discrete variable,the activation of a promoter, is prone to errors due to leakiness and cross-talk between HSL molecules, regulatory proteins and promoters (we characterized it for Lux promoter (BBa_R0062), Las promoter (BBa_R0079) and Rhl promoter (BBa_R071)). To limit the risk of error propagation throughout the rows, we used a riboregulating system.

Computing

Integrases compute an XOR logic gate via single switching or double switching of a terminator. Their behavior depends on a set of parameters.

Producing =

Difference between cell red and cell blue.

Sending

The HSL produced diffuses then through the channels to the rows below. The speed of diffusion of each HSL determines coordination on the chip and the time needed from the pattern to emerge. You can find out more information about diffusion on our modeling page, our experimental results page and our chip page.

Coordination is necessary on the chip.