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Team:ETH Zurich/project/infopro
From 2014.igem.org
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=== Initating The Signal === | === Initating The Signal === | ||
| - | We initiate the signal by pipetting one sort of HSL in a well of the first line. | + | We initiate the signal by pipetting one sort of 3OC6-HSL in a well of the first line. |
=== Sensing === | === Sensing === | ||
| - | Quorum | + | Diffused HSL molecules are identified via [https://2014.igem.org/Team:ETH_Zurich/expresults#Quorum quorum sensing]. This [https://2014.igem.org/Team:ETH_Zurich/modeling/qs integration] from a continuous variable, the concentration of HSL, to a discrete variable,the activation of a promoter, is prone to errors due to leakiness and cross-talk between HSL molecules, regulatory proteins and promoters (we characterized it for [http://parts.igem.org/Part:BBa_R0062 Lux promoter (BBa_R0062)], [http://parts.igem.org/Part:BBa_R0079 Las promoter (BBa_R0079)] and [http://parts.igem.org/Part:BBa_R0071 Rhl promoter (BBa_R071)]). To limit the risk of error propagation throughout the rows, we used a [http://parts.igem.org/Part:BBa_K1541000 riboregulating system]. |
=== Computing === | === Computing === | ||
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=== Sending === | === Sending === | ||
| + | |||
| + | The HSL produced diffuses then through the channels to the rows below. The speed of diffusion of each HSL determines coordination on the chip. You can find out more information about diffusion on our modeling page, our experimental results page and our chip page. | ||
Diffusion | Diffusion | ||
[[File:ETH_Zurich_Information_Processing_chip.png|center|800px|thumb|Coordination is necessary on the chip.]] | [[File:ETH_Zurich_Information_Processing_chip.png|center|800px|thumb|Coordination is necessary on the chip.]] | ||
| - | + | ||
<html></article></html> | <html></article></html> | ||
{{:Team:ETH_Zurich/tpl/foot}} | {{:Team:ETH_Zurich/tpl/foot}} | ||
Revision as of 19:48, 11 October 2014
Information Processing
Global Information Processing Pathway
Initating The Signal
We initiate the signal by pipetting one sort of 3OC6-HSL in a well of the first line.
Sensing
Diffused HSL molecules are identified via quorum sensing. This integration from a continuous variable, the concentration of HSL, to a discrete variable,the activation of a promoter, is prone to errors due to leakiness and cross-talk between HSL molecules, regulatory proteins and promoters (we characterized it for Lux promoter (BBa_R0062), Las promoter (BBa_R0079) and Rhl promoter (BBa_R071)). To limit the risk of error propagation throughout the rows, we used a riboregulating system.
Computing
Integrases: Modeling ==> Integrating a signal
Producing =
Difference between cell red and cell blue.
Sending
The HSL produced diffuses then through the channels to the rows below. The speed of diffusion of each HSL determines coordination on the chip. You can find out more information about diffusion on our modeling page, our experimental results page and our chip page.
Diffusion
