Team:ETH Zurich/project/infopro
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[https://2014.igem.org/Team:ETH_Zurich/expresults#Integrases Integrases] compute an [https://2014.igem.org/Team:ETH_Zurich/modeling/xor XOR logic gate] via single switching or double switching of a terminator. Their behavior depends on a set of [https://2014.igem.org/Team:ETH_Zurich/modeling/int parameters]. | [https://2014.igem.org/Team:ETH_Zurich/expresults#Integrases Integrases] compute an [https://2014.igem.org/Team:ETH_Zurich/modeling/xor XOR logic gate] via single switching or double switching of a terminator. Their behavior depends on a set of [https://2014.igem.org/Team:ETH_Zurich/modeling/int parameters]. | ||
- | === | + | === Postprocessing === |
- | + | The enzyme synthesizes the signaling molecule HSL. | |
=== Sending === | === Sending === |
Revision as of 12:01, 16 October 2014
Information Processing
Initating The Signal
We initiate the signal by pipetting 3OC6-HSL in a well of the first line.
Sensing
Diffused HSL molecules are identified via quorum sensing. This integration from a continuous variable, the concentration of HSL, to a discrete variable, the activation of a promoter, is prone to errors due to leakiness and crosstalk between HSL molecules, regulatory proteins and promoters (we characterized it for Lux promoter (BBa_R0062), Las promoter (BBa_R0079) and Rhl promoter (BBa_R0071)). To limit the risk of error propagation throughout the rows, we used a riboregulating system.
Computing
Integrases compute an XOR logic gate via single switching or double switching of a terminator. Their behavior depends on a set of parameters.
Postprocessing
The enzyme synthesizes the signaling molecule HSL.
Sending
The HSL produced diffuses then through the channels to the rows below. The speed of this directed diffusion determines the time needed from the pattern to emerge. You can find out more information about diffusion and chip design on our modeling page, our experimental results page and our chip page.