Team:ETH Zurich/project/infopro

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(The goal: Emergence of patterns via information processing)
(The goal: Emergence of patterns via information processing)
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=== The goal: Emergence of patterns via information processing===
=== The goal: Emergence of patterns via information processing===
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We implement a cellular automaton with bacterial cells containing our logic circuitry. Each bacterial colony serves as a core, computing an XOR gate. First, a sensor device detects the input, ''N''-acyl homoserine lactones (AHL). Then, the cell integrates this signal through a logic gate, performed by serine integrases by sensing on the protein level and acting on the DNA level. A necessary post-processing step allows then the production of a new AHL variant due to activated gene expression through the integrase. Meanwhile,  green fluorescent protein (GFP) indicates the state of the colony and serves as a long-lasting visual read out. The produced AHL output-signal then propagates in a directed fashion through a millifluidic grid to the next bacterial colony. This iterative process faces the challenges of leakiness, cross-talk, protein-level computation and exact diffusion steps.
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We implement a cellular automaton with bacterial cells containing our logic circuitry. Each bacterial colony serves as a core, computing an XOR gate. First, a sensor device detects the input, ''N''-acyl homoserine lactones (AHL). Then, the cell integrates this signal through a logic gate, performed by serine integrases by sensing on the protein level and acting on the DNA level. A necessary post-processing step allows then the production of a new AHL variant due to activated gene expression through the integrase. Meanwhile,  green fluorescent protein (GFP) indicates the state of the colony and serves as a long-lasting visual read out. The produced AHL output-signal then propagates in a directed fashion through a millifluidic grid to the next bacterial colony. This iterative process faces the challenges of leakiness, cross-talk, protein-level computation and exact diffusion steps. this information pathway is shown in figure 1.

Revision as of 02:22, 18 October 2014

iGEM ETH Zurich 2014