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Team:ETH Zurich/project/infopro
From 2014.igem.org
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[[File:ETH_Zurich_Information_Processing.png|center|800px]] | [[File:ETH_Zurich_Information_Processing.png|center|800px]] | ||
| - | == Initating The Signal == | + | === Initating The Signal === |
| - | We initiate the signal by pipetting | + | We initiate the signal by pipetting 3OC6-HSL in a well of the first line. |
| - | == Sensing == | + | === Sensing === |
Diffused HSL molecules are identified via [https://2014.igem.org/Team:ETH_Zurich/expresults#Quorum quorum sensing]. This [https://2014.igem.org/Team:ETH_Zurich/modeling/qs integration] from a continuous variable, the concentration of HSL, to a discrete variable,the activation of a promoter, is prone to errors due to leakiness and cross-talk between HSL molecules, regulatory proteins and promoters (we characterized it for [http://parts.igem.org/Part:BBa_R0062 Lux promoter (BBa_R0062)], [http://parts.igem.org/Part:BBa_R0079 Las promoter (BBa_R0079)] and [http://parts.igem.org/Part:BBa_R0071 Rhl promoter (BBa_R071)]). To limit the risk of error propagation throughout the rows, we used a [http://parts.igem.org/Part:BBa_K1541000 riboregulating system]. | Diffused HSL molecules are identified via [https://2014.igem.org/Team:ETH_Zurich/expresults#Quorum quorum sensing]. This [https://2014.igem.org/Team:ETH_Zurich/modeling/qs integration] from a continuous variable, the concentration of HSL, to a discrete variable,the activation of a promoter, is prone to errors due to leakiness and cross-talk between HSL molecules, regulatory proteins and promoters (we characterized it for [http://parts.igem.org/Part:BBa_R0062 Lux promoter (BBa_R0062)], [http://parts.igem.org/Part:BBa_R0079 Las promoter (BBa_R0079)] and [http://parts.igem.org/Part:BBa_R0071 Rhl promoter (BBa_R071)]). To limit the risk of error propagation throughout the rows, we used a [http://parts.igem.org/Part:BBa_K1541000 riboregulating system]. | ||
| - | == Computing == | + | === Computing === |
[https://2014.igem.org/Team:ETH_Zurich/expresults#Integrases Integrases] compute an [https://2014.igem.org/Team:ETH_Zurich/modeling/xor XOR logic gate] via single switching or double switching of a terminator. Their behavior depends on a set of [https://2014.igem.org/Team:ETH_Zurich/modeling/int parameters]. | [https://2014.igem.org/Team:ETH_Zurich/expresults#Integrases Integrases] compute an [https://2014.igem.org/Team:ETH_Zurich/modeling/xor XOR logic gate] via single switching or double switching of a terminator. Their behavior depends on a set of [https://2014.igem.org/Team:ETH_Zurich/modeling/int parameters]. | ||
| - | == Producing == | + | === Producing === |
As a readable output of the logic gate, fast-folding GFP is produced, as well as an enzyme synthetizing HSL to be diffused to the next level. | As a readable output of the logic gate, fast-folding GFP is produced, as well as an enzyme synthetizing HSL to be diffused to the next level. | ||
| - | == Sending == | + | === Sending === |
The HSL produced diffuses then through the channels to the rows below. The speed of diffusion of each HSL determines coordination on the chip and the time needed from the pattern to emerge. You can find out more information about diffusion on our [https://2014.igem.org/Team:ETH_Zurich/modeling/diffmodel modeling page], our [https://2014.igem.org/Team:ETH_Zurich/expresults#Diffusion experimental results page] and our [https://2014.igem.org/Team:ETH_Zurich/lab/chip chip page]. | The HSL produced diffuses then through the channels to the rows below. The speed of diffusion of each HSL determines coordination on the chip and the time needed from the pattern to emerge. You can find out more information about diffusion on our [https://2014.igem.org/Team:ETH_Zurich/modeling/diffmodel modeling page], our [https://2014.igem.org/Team:ETH_Zurich/expresults#Diffusion experimental results page] and our [https://2014.igem.org/Team:ETH_Zurich/lab/chip chip page]. | ||
Revision as of 20:19, 11 October 2014
Information Processing
Initating The Signal
We initiate the signal by pipetting 3OC6-HSL in a well of the first line.
Sensing
Diffused HSL molecules are identified via quorum sensing. This integration from a continuous variable, the concentration of HSL, to a discrete variable,the activation of a promoter, is prone to errors due to leakiness and cross-talk between HSL molecules, regulatory proteins and promoters (we characterized it for Lux promoter (BBa_R0062), Las promoter (BBa_R0079) and Rhl promoter (BBa_R071)). To limit the risk of error propagation throughout the rows, we used a riboregulating system.
Computing
Integrases compute an XOR logic gate via single switching or double switching of a terminator. Their behavior depends on a set of parameters.
Producing
As a readable output of the logic gate, fast-folding GFP is produced, as well as an enzyme synthetizing HSL to be diffused to the next level.
Sending
The HSL produced diffuses then through the channels to the rows below. The speed of diffusion of each HSL determines coordination on the chip and the time needed from the pattern to emerge. You can find out more information about diffusion on our modeling page, our experimental results page and our chip page.
