Team:ETH Zurich/modeling/xor

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In our design, we are interested in a double flipping. That is to say that two pairs of binding sites surrounds the fragment to be flipped. One pair of binding sites can be bound by DBxb1 and the other one by ΦC31.
In our design, we are interested in a double flipping. That is to say that two pairs of binding sites surrounds the fragment to be flipped. One pair of binding sites can be bound by DBxb1 and the other one by ΦC31.
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=== Reactions ===
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$$\begin{align*}
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SA_{Bxb1}+SA_{Bxb1}+T_{on,i}& \rightarrow T_{offBxb1}+ SF_{Bxb1}+SF_{Bxb1}\\
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T_{offBxb1} &\rightarrow T_{offBxb1} + mRNA_{GFP} + mRNA_{LasI} \\
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SA_{\phi C31}+SA_{\phi C31}+T_{on,i}& \rightarrow T_{off\phi C31}+SF_{\phi C31}+SF_{\phi C31}\\
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T_{off\phi C31} &\rightarrow T_{off\phi C31} + mRNA_{GFP} + mRNA_{LasI} \\
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SA_{Bxb1}+SA_{Bxb1}+T_{off,\phi C31}& \rightarrow T_{on,f}+SF_{Bxb1}+SF_{Bxb1}\\
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SA_{\phi C31}+SA_{\phi C31}+T_{offBxb1}& \rightarrow T_{on,f}+SF_{\phi C31}+SF_{\phi C31}\\
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mRNA_{GFP} &\rightarrow GFP\\
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mRNA_{LasI} &\rightarrow LasI\\
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mRNA_{GFP} &\rightarrow \emptyset\\
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mRNA_{LasI}&\rightarrow \emptyset\\
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GFP &\rightarrow \emptyset\\
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LasI&\rightarrow \emptyset
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\end{align*}$$
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Revision as of 11:26, 13 October 2014

iGEM ETH Zurich 2014