Team:ETH Zurich/modeling/whole

From 2014.igem.org

(Difference between revisions)
(With Leakiness and Crosstalk)
(With Leakiness and Crosstalk)
Line 287: Line 287:
As all the measurements were in terms of fluorescence, the V<sub>max</sub> for each Hill function was also in terms of fluorescence. In order to observe the effect of cross-talk we normalized the V<sub>max</sub> of non native interactions (in Lux system, P<sub>Lux</sub> being activated by LasAHL-LuxR, LasAHL-LasR or LuxAHL-LasR complexes) with the V<sub>max</sub> of the native interaction (LuxR binding to LuxAHL and activating P<sub>Lux</sub>). These ratios acted as weights for the effect of a non-native interaction on the promoter.  
As all the measurements were in terms of fluorescence, the V<sub>max</sub> for each Hill function was also in terms of fluorescence. In order to observe the effect of cross-talk we normalized the V<sub>max</sub> of non native interactions (in Lux system, P<sub>Lux</sub> being activated by LasAHL-LuxR, LasAHL-LasR or LuxAHL-LasR complexes) with the V<sub>max</sub> of the native interaction (LuxR binding to LuxAHL and activating P<sub>Lux</sub>). These ratios acted as weights for the effect of a non-native interaction on the promoter.  
 +
 +
[[File:ETHZ_DynamicsWithCrossTalk.png|center|800px|thumb|'''Figure 7''' The four cases for the whole cell with basal leakiness and cross-talk]]
 +
For an ideal whole cell model, it was necessary  to have no or minimum leakiness and cross-talk. However, in reality this was not the case. Although we were able to reduce leakiness significantly using riboregulators, we still had the issue of cross-talk. However, we observe that the system acts as an XOR gate till about 300 mins even with cross talk.  
For an ideal whole cell model, it was necessary  to have no or minimum leakiness and cross-talk. However, in reality this was not the case. Although we were able to reduce leakiness significantly using riboregulators, we still had the issue of cross-talk. However, we observe that the system acts as an XOR gate till about 300 mins even with cross talk.  
Line 292: Line 295:
-
[[File:ETHZ_DynamicsWithCrossTalk.png|center|800px|thumb|'''Figure 7''' The four cases for the whole cell with basal leakiness and cross-talk]]
+
[[File:ETHZ_WikiXORCTDiffLeakiness.png|center|500px|thumb|'''Figure 7''' The four cases for the whole cell with basal leakiness and cross-talk]]
 +
 
<html></article></html>
<html></article></html>

Revision as of 01:48, 18 October 2014

iGEM ETH Zurich 2014