Team:ETH Zurich/modeling/qs

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== Leakiness ==
== Leakiness ==
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The leakiness of promoters is a major issue in our system. As the signal propagates row-wise, error diffusion could lead to a totally different pattern. The goal is then to master the leakiness. This issue was particularly observed in the case of the Lux promoter during our [https://2014.igem.org/Team:ETH_Zurich/expresults experiments set]. This leakiness is dependent on the LuxR concentration in the cell(see the [http://parts.igem.org/Part:BBa_R0062:Experience Registry] for more information).
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The leakiness of promoters is a major issue in our system. As the signal propagates row-wise, error diffusion could lead to a totally different pattern. The goal is then to control the leakiness. This issue was particularly observed in the case of the Lux promoter during our [https://2014.igem.org/Team:ETH_Zurich/expresults experiments set]. This leakiness is dependent on the LuxR concentration in the cell(see the [http://parts.igem.org/Part:BBa_R0062:Experience Registry] for more information).
Leakiness was modeled as an offset in the classical Hill function.  
Leakiness was modeled as an offset in the classical Hill function.  
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Given this offset and the maximal expression, the signal over noise ratio can be derived. This ratio, which comparable amongst all curves, characterizes the impact of leakiness on the behavior of a system. Our final construct (Promoters with a riboregulating system) have the following parameters.
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Given this offset and the maximal expression, the signal over noise ratio can be derived. This ratio, which comparable amongst all curves, characterizes the impact of leakiness on the behavior of a system. Our final construct (Promoters with a [https://2014.igem.org/Team:ETH_Zurich/expresults riboregulating system]) have the following parameters.
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%%Insert table%%
We took the leakiness coefficients into account in our [https://2014.igem.org/Team:ETH_Zurich/modeling/whole whole cell model].
We took the leakiness coefficients into account in our [https://2014.igem.org/Team:ETH_Zurich/modeling/whole whole cell model].

Revision as of 07:47, 17 October 2014

iGEM ETH Zurich 2014