# Team:ETH Zurich/modeling/qs

(Difference between revisions)
 Revision as of 06:39, 17 October 2014 (view source)Eledieu (Talk | contribs)m (→Cross-talk)← Older edit Revision as of 07:10, 17 October 2014 (view source)Eledieu (Talk | contribs) m (→Cross-talk)Newer edit → Line 237: Line 237: == Cross-talk == == Cross-talk == - We investigated the existence of cross-talk between three quorum sensing systems (Lux, Las and Rhl). From the [http://2014.igem.org/Team:ETH_Zurich/expresults experimental data], two-orders of cross-talk were identified. + We investigated the existence of cross-talk between three quorum sensing systems (Lux, Las and Rhl). Cross-talk implies non-orthogonality of the communication systems. It corresponds to the fact that LasAHL can activate the Lux promoter, even if it is not its native communicating pathway. [[File:ETH Zurich Crosstalk.png|1500px|center|thumb|Each quorum sensing system is based on three components: a signaling molecule, a regulatory protein and a promoter. These elements are here ordered into three layers. Cross-talk evaluation can be done by comparing all combinations of those three elements. After collecting the [http://2014.igem.org/Team:ETH_Zurich/expresults experimental data] of all possible pathways, we modeled their influence.]] [[File:ETH Zurich Crosstalk.png|1500px|center|thumb|Each quorum sensing system is based on three components: a signaling molecule, a regulatory protein and a promoter. These elements are here ordered into three layers. Cross-talk evaluation can be done by comparing all combinations of those three elements. After collecting the [http://2014.igem.org/Team:ETH_Zurich/expresults experimental data] of all possible pathways, we modeled their influence.]] + + From the [http://2014.igem.org/Team:ETH_Zurich/expresults exhaustive experimental data], the central role of regulatory proteins was identified, allowing the characterization of two-levels of cross-talk. The following parts in the Registry are presenting our results: + *[http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062] + *[http://parts.igem.org/Part:BBa_R0079:Experience BBa_R0079] + *[http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017] + *[http://parts.igem.org/Part:BBa_C0062:Experience BBa_C0062] + *[http://parts.igem.org/Part:BBa_C0179:Experience BBa_C0179] + *[http://parts.igem.org/Part:BBa_C0171:Experience BBa_C0171] + + Each experimental data set was fitted to an Hill function using the Least Absolute Residual method. + + $$rFluo = a + b \frac{[AHL]^n}{K_m^n + [AHL]^n} + where rFluo is the relative fluorescence (absolute measured fluorescence value over OD), + a the basal expression rate + b the maximum fold expression rate + n the Hill coeeficient + K_m the activation concentration of AHL$$

## Revision as of 07:10, 17 October 2014

iGEM ETH Zurich 2014