Team:ETH Zurich/modeling/qs

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== Cross-talk ==
== Cross-talk ==
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We investigated the existence of cross-talk between three quorum sensing systems (Lux, Las and Rhl). From the [http://2014.igem.org/Team:ETH_Zurich/expresults experimental data], two-orders of cross-talk were identified.
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We investigated the existence of cross-talk between three quorum sensing systems (Lux, Las and Rhl). Cross-talk implies non-orthogonality of the communication systems. It corresponds to the fact that LasAHL can activate the Lux promoter, even if it is not its native communicating pathway.
[[File:ETH Zurich Crosstalk.png|1500px|center|thumb|Each quorum sensing system is based on three components: a signaling molecule, a regulatory protein and a promoter. These elements are here ordered into three layers. Cross-talk evaluation can be done by comparing all combinations of those three elements. After collecting the [http://2014.igem.org/Team:ETH_Zurich/expresults experimental data] of all possible pathways, we modeled their influence.]]
[[File:ETH Zurich Crosstalk.png|1500px|center|thumb|Each quorum sensing system is based on three components: a signaling molecule, a regulatory protein and a promoter. These elements are here ordered into three layers. Cross-talk evaluation can be done by comparing all combinations of those three elements. After collecting the [http://2014.igem.org/Team:ETH_Zurich/expresults experimental data] of all possible pathways, we modeled their influence.]]
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From the [http://2014.igem.org/Team:ETH_Zurich/expresults exhaustive experimental data], the central role of regulatory proteins was identified, allowing the characterization of two-levels of cross-talk. The following parts in the Registry are presenting our results:
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*[http://parts.igem.org/Part:BBa_R0062:Experience BBa_R0062]
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*[http://parts.igem.org/Part:BBa_R0079:Experience BBa_R0079]
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*[http://parts.igem.org/Part:BBa_I14017:Experience BBa_I14017]
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*[http://parts.igem.org/Part:BBa_C0062:Experience BBa_C0062]
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*[http://parts.igem.org/Part:BBa_C0179:Experience BBa_C0179]
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*[http://parts.igem.org/Part:BBa_C0171:Experience BBa_C0171]
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Each experimental data set was fitted to an Hill function using the Least Absolute Residual method.
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$$ rFluo = a + b \frac{[AHL]^n}{K_m^n + [AHL]^n}
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where rFluo is the relative fluorescence (absolute measured fluorescence value over OD),
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a the basal expression rate
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b the maximum fold expression rate
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n the Hill coeeficient
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K_m the activation concentration of AHL$$
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Revision as of 07:10, 17 October 2014

iGEM ETH Zurich 2014