Team:ETH Zurich/modeling/parameters
From 2014.igem.org
Parameters
No model is complete without parameters. Our exhaustive list of parameters are summarised in the table below.
Parameter | Value | Description | Reference | |||||
---|---|---|---|---|---|---|---|---|
αLuxR | 60 nMmin-1 | Production rate of LuxR | Estimated | |||||
kRLux | 0.1 nM-1min-1 | Rate of formation of RLux from 30C6HSL and LuxR | Weber | |||||
k-RLux | 10 min-1 | Dissociation rate of RLux | Weber | |||||
kDRLux | 0.05 nM-1min-1 | Dimerization rate of RLux | Weber | |||||
k-DRLux | 1 min-1 | Dissociation rate of DRLux | Weber | |||||
KdPLux | 0.007652 nM | Dissociation rate constant for DRLux-PLux | Fitted | |||||
d30C6HSL | 0.004 min-1 | Degradation rate of 30C6HSL | Estimated/Fitted | |||||
dLuxR | 0.0174 min-1 | Degradation rate of LuxR | Weber | |||||
dRLux | 0.0174 min-1 | Degradation rate of RLux | Weber | |||||
dDRLux | 0.0231 min-1 | Degradation rate of DRLux | Weber | |||||
dBxb1 | 0.01 min-1 | Degradation rate of Bxb1 | Assumed | |||||
LPLux | 0.04458 nM-1min-1 | Leakiness after using riboswitch for PLux | Fitted | |||||
αLasR | 60 nMmin-1 | Production rate of LasR | Estimated | |||||
kRLas | 0.1 nM-1min-1 | Rate of formation of RLas from 30C12HSL and LasR | Weber | |||||
k-RLas | 10 min-1 | Dissociation rate of RLas | Weber | |||||
kDRLas | 0.05 nM-1min-1 | Dimerization rate of RLas | Weber | |||||
k-DRLas | 1 min-1 | Dissociation rate of DRLas | Weber | |||||
KdPLas | 1.3672 nM | Dissociation rate constant for DRLas-PLas | Fitted | |||||
d30C12HSL | 0.004 min-1 | Degradation rate of 30C12HSL | Estimated/Fitted | |||||
dLasR | 0.0174 min-1 | Degradation rate of LasR | Weber | |||||
dRLas | 0.0174 min-1 | Degradation rate of RLas | Weber | |||||
dDRLas | 0.0231 min-1 | Degradation rate of DRLas | Weber | |||||
dϕC31 | 0.01 min-1 | Degradation rate of ϕC31 | Assumed | |||||
LPLux | 0.02461 nM-1min-1 | Leakiness after using riboswitch for PLas | Fitted | |||||
kDBxb1 | 1 nM-1min-1 | Dimerization rate of Bxb1 | Fitted | |||||
k-DBxb1 | 10-4 min-1 | Dissociation rate of DBxb1 | Fitted | |||||
kSABxb1 | 1 nM-1min-1 | Rate of formation of SABxb1 from DBxb1 and SIBxb1 | Fitted | |||||
k-SABxb1 | 10-4 min-1 | Dissociation rate of SABxb1 | Fitted | |||||
dDBxb1 | 0.02 min-1 | Degradation rate of DBxb1 | Assumed | |||||
kϕc31 | 1 nM-1min-1 | Dimerization rate of ϕc31 | Fitted | |||||
k-Dϕc31 | 10-4 min-1 | Rate of dissociation of Dϕc31 | Fitted | |||||
kSAϕc31 | 1 nM-1min-1 | Rate of formation of SAϕc31 from Dϕc31 and SIϕc31 | Fitted | |||||
k-SAϕc31 | 10-4 min-1 | Rate of dissociation of SAϕc31 | Fitted | |||||
dDϕc31 | 0.02 min-1 | Degradation rate of Dϕc31 | Assumed | |||||
koffBxb1 | 100 nM-2min-1 | Rate of flipping of Ton,i to ToffBxb1 | Assumed | |||||
kGFP,LuxI | 10 min-1 | Production rate of GFP and LuxI | Assumed | k-offBxb1 | 100 nM-2min-1 | Rate of flipping of ToffBxb1 to Ton,f | Assumed |