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Team:ETH Zurich/modeling/parameters
From 2014.igem.org
(Difference between revisions)
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|d<sub>Bxb1</sub>||0.01 min<sup>-1</sup>||Degradation rate of Bxb1||Assumed | |d<sub>Bxb1</sub>||0.01 min<sup>-1</sup>||Degradation rate of Bxb1||Assumed | ||
|- | |- | ||
| - | |L<sub>PLux</sub>||0.04458 nM<sup>-1</sup>min<sup>-1</sup>||Leakiness after using riboswitch||Fitted | + | |L<sub>PLux</sub>||0.04458 nM<sup>-1</sup>min<sup>-1</sup>||Leakiness after using riboswitch for PLux||Fitted |
|- | |- | ||
|α<sub>LasR</sub>||60 nMmin<sup>-1</sup>||Production rate of LasR||Estimated | |α<sub>LasR</sub>||60 nMmin<sup>-1</sup>||Production rate of LasR||Estimated | ||
|- | |- | ||
| - | |k<sub>RLas</sub>||0.1 nM<sup>-1</sup>min<sup>-1</sup>|| Rate of formation of RLas from | + | |k<sub>RLas</sub>||0.1 nM<sup>-1</sup>min<sup>-1</sup>|| Rate of formation of RLas from 30C12HSL and LasR||Weber |
|- | |- | ||
|k<sub>-RLas</sub>||10 min<sup>-1</sup>||Dissociation rate of RLas ||Weber | |k<sub>-RLas</sub>||10 min<sup>-1</sup>||Dissociation rate of RLas ||Weber | ||
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|K<sub>dPLas</sub>||1.3672 nM||Dissociation rate constant for DRLas-PLas ||Fitted | |K<sub>dPLas</sub>||1.3672 nM||Dissociation rate constant for DRLas-PLas ||Fitted | ||
|- | |- | ||
| - | |d<sub> | + | |d<sub>30C12HSL</sub>||0.004 min<sup>-1</sup>||Degradation rate of 30C12HSL||Estimated/Fitted |
|- | |- | ||
|d<sub>LasR</sub>||0.0174 min<sup>-1</sup>||Degradation rate of LasR||Weber | |d<sub>LasR</sub>||0.0174 min<sup>-1</sup>||Degradation rate of LasR||Weber | ||
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|- | |- | ||
|d<sub>ϕC31</sub>||0.01 min<sup>-1</sup>||Degradation rate of ϕC31||Assumed | |d<sub>ϕC31</sub>||0.01 min<sup>-1</sup>||Degradation rate of ϕC31||Assumed | ||
| - | + | |- | |
| + | |L<sub>PLux</sub>||0.02461 nM<sup>-1</sup>min<sup>-1</sup>||Leakiness after using riboswitch for PLas||Fitted | ||
|} | |} | ||
{{:Team:ETH Zurich/tpl/foot}} | {{:Team:ETH Zurich/tpl/foot}} | ||
Revision as of 03:58, 9 October 2014
Parameters
No model is complete without parameters. Our exhaustive list of parameters are summarised in the table below.
| Parameter | Value | Description | Reference |
|---|---|---|---|
| αLuxR | 60 nMmin-1 | Production rate of LuxR | Estimated |
| kRLux | 0.1 nM-1min-1 | Rate of formation of RLux from 30C6HSL and LuxR | Weber |
| k-RLux | 10 min-1 | Dissociation rate of RLux | Weber |
| kDRLux | 0.05 nM-1min-1 | Dimerization rate of RLux | Weber |
| k-DRLux | 1 min-1 | Dissociation rate of DRLux | Weber |
| KdPLux | 0.007652 nM | Dissociation rate constant for DRLux-PLux | Fitted |
| d30C6HSL | 0.004 min-1 | Degradation rate of 30C6HSL | Estimated/Fitted |
| dLuxR | 0.0174 min-1 | Degradation rate of LuxR | Weber |
| dRLux | 0.0174 min-1 | Degradation rate of RLux | Weber |
| dDRLux | 0.0231 min-1 | Degradation rate of DRLux | Weber |
| dBxb1 | 0.01 min-1 | Degradation rate of Bxb1 | Assumed |
| LPLux | 0.04458 nM-1min-1 | Leakiness after using riboswitch for PLux | Fitted |
| αLasR | 60 nMmin-1 | Production rate of LasR | Estimated |
| kRLas | 0.1 nM-1min-1 | Rate of formation of RLas from 30C12HSL and LasR | Weber |
| k-RLas | 10 min-1 | Dissociation rate of RLas | Weber |
| kDRLas | 0.05 nM-1min-1 | Dimerization rate of RLas | Weber |
| k-DRLas | 1 min-1 | Dissociation rate of DRLas | Weber |
| KdPLas | 1.3672 nM | Dissociation rate constant for DRLas-PLas | Fitted |
| d30C12HSL | 0.004 min-1 | Degradation rate of 30C12HSL | Estimated/Fitted |
| dLasR | 0.0174 min-1 | Degradation rate of LasR | Weber |
| dRLas | 0.0174 min-1 | Degradation rate of RLas | Weber |
| dDRLas | 0.0231 min-1 | Degradation rate of DRLas | Weber |
| dϕC31 | 0.01 min-1 | Degradation rate of ϕC31 | Assumed |
| LPLux | 0.02461 nM-1min-1 | Leakiness after using riboswitch for PLas | Fitted |
