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Team:ETH Zurich/modeling/parameters
From 2014.igem.org
(Difference between revisions)
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|d<sub>Bxb1</sub>||0.01 min<sup>-1</sup>||Degradation rate of Bxb1||Assumed | |d<sub>Bxb1</sub>||0.01 min<sup>-1</sup>||Degradation rate of Bxb1||Assumed | ||
| + | |- | ||
| + | |L<sub>PLux</sub>||0.04458 nM<sup>-1</sup>min<sup>-1</sup>||Leakiness after using riboswitch||Fitted | ||
|- | |- | ||
|α<sub>LasR</sub>||60 nMmin<sup>-1</sup>||Production rate of LasR||Estimated | |α<sub>LasR</sub>||60 nMmin<sup>-1</sup>||Production rate of LasR||Estimated | ||
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|d<sub>DRLas</sub>||0.0231 min<sup>-1</sup>||Degradation rate of DRLas||Weber | |d<sub>DRLas</sub>||0.0231 min<sup>-1</sup>||Degradation rate of DRLas||Weber | ||
|- | |- | ||
| - | |d<sub> | + | |d<sub>ϕC31</sub>||0.01 min<sup>-1</sup>||Degradation rate of ϕC31||Assumed |
!Total: 15!!!!!!Total: 29.55!! | !Total: 15!!!!!!Total: 29.55!! | ||
|} | |} | ||
{{:Team:ETH Zurich/tpl/foot}} | {{:Team:ETH Zurich/tpl/foot}} | ||
Revision as of 03:55, 9 October 2014
Parameters
No model is complete without parameters. Our exhaustive list of parameters are summarised in the table below.
| Parameter | Value | Description | Reference | |||||
|---|---|---|---|---|---|---|---|---|
| αLuxR | 60 nMmin-1 | Production rate of LuxR | Estimated | |||||
| kRLux | 0.1 nM-1min-1 | Rate of formation of RLux from 30C6HSL and LuxR | Weber | |||||
| k-RLux | 10 min-1 | Dissociation rate of RLux | Weber | |||||
| kDRLux | 0.05 nM-1min-1 | Dimerization rate of RLux | Weber | |||||
| k-DRLux | 1 min-1 | Dissociation rate of DRLux | Weber | |||||
| KdPLux | 0.007652 nM | Dissociation rate constant for DRLux-PLux | Fitted | |||||
| d30C6HSL | 0.004 min-1 | Degradation rate of 30C6HSL | Estimated/Fitted | |||||
| dLuxR | 0.0174 min-1 | Degradation rate of LuxR | Weber | |||||
| dRLux | 0.0174 min-1 | Degradation rate of RLux | Weber | |||||
| dDRLux | 0.0231 min-1 | Degradation rate of DRLux | Weber | |||||
| dBxb1 | 0.01 min-1 | Degradation rate of Bxb1 | Assumed | |||||
| LPLux | 0.04458 nM-1min-1 | Leakiness after using riboswitch | Fitted | |||||
| αLasR | 60 nMmin-1 | Production rate of LasR | Estimated | |||||
| kRLas | 0.1 nM-1min-1 | Rate of formation of RLas from 30C6HSL and LasR | Weber | |||||
| k-RLas | 10 min-1 | Dissociation rate of RLas | Weber | |||||
| kDRLas | 0.05 nM-1min-1 | Dimerization rate of RLas | Weber | |||||
| k-DRLas | 1 min-1 | Dissociation rate of DRLas | Weber | |||||
| KdPLas | 1.3672 nM | Dissociation rate constant for DRLas-PLas | Fitted | |||||
| d30C6HSL | 0.004 min-1 | Degradation rate of 30C6HSL | Estimated/Fitted | |||||
| dLasR | 0.0174 min-1 | Degradation rate of LasR | Weber | |||||
| dRLas | 0.0174 min-1 | Degradation rate of RLas | Weber | |||||
| dDRLas | 0.0231 min-1 | Degradation rate of DRLas | Weber | |||||
| dϕC31 | 0.01 min-1 | Degradation rate of ϕC31 | Assumed | Total: 15 | Total: 29.55 |
