Team:ETH Zurich/modeling/parameters
From 2014.igem.org
(Difference between revisions)
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|k<sub>-DRLux</sub>||1 min<sup>-1</sup>||Dissociation rate of DRLux ||Weber | |k<sub>-DRLux</sub>||1 min<sup>-1</sup>||Dissociation rate of DRLux ||Weber | ||
|- | |- | ||
- | |K<sub>dPLux</sub>||0.007652 | + | |K<sub>dPLux</sub>||0.007652 nM||Dissociation rate constant for DRLux-PLux ||Fitted |
|- | |- | ||
|d<sub>30C6HSL</sub>||0.004 min<sup>-1</sup>||Degradation rate of 30C6HSL||Estimated/Fitted | |d<sub>30C6HSL</sub>||0.004 min<sup>-1</sup>||Degradation rate of 30C6HSL||Estimated/Fitted | ||
Line 29: | Line 29: | ||
|- | |- | ||
|d<sub>Bxb1</sub>||0.01 min<sup>-1</sup>||Degradation rate of Bxb1||Assumed | |d<sub>Bxb1</sub>||0.01 min<sup>-1</sup>||Degradation rate of Bxb1||Assumed | ||
+ | |- | ||
+ | |α<sub>LasR</sub>||60 nMmin<sup>-1</sup>||Production rate of LasR||Estimated | ||
+ | |- | ||
+ | |k<sub>RLas</sub>||0.1 nM<sup>-1</sup>min<sup>-1</sup>|| Rate of formation of RLas from 30C6HSL and LasR||Weber | ||
+ | |- | ||
+ | |k<sub>-RLas</sub>||10 min<sup>-1</sup>||Dissociation rate of RLas ||Weber | ||
+ | |- | ||
+ | |k<sub>DRLas</sub>||0.05 nM<sup>-1</sup>min<sup>-1</sup>||Dimerization rate of RLas ||Weber | ||
+ | |- | ||
+ | |k<sub>-DRLas</sub>||1 min<sup>-1</sup>||Dissociation rate of DRLas ||Weber | ||
+ | |- | ||
+ | |K<sub>dPLas</sub>||1.3672 nM||Dissociation rate constant for DRLas-PLas ||Fitted | ||
+ | |- | ||
+ | |d<sub>30C6HSL</sub>||0.004 min<sup>-1</sup>||Degradation rate of 30C6HSL||Estimated/Fitted | ||
+ | |- | ||
+ | |d<sub>LasR</sub>||0.0174 min<sup>-1</sup>||Degradation rate of LasR||Weber | ||
+ | |- | ||
+ | |d<sub>RLas</sub>||0.0174 min<sup>-1</sup>||Degradation rate of RLas||Weber | ||
+ | |- | ||
+ | |d<sub>DRLas</sub>||0.0231 min<sup>-1</sup>||Degradation rate of DRLas||Weber | ||
+ | |- | ||
+ | |d<sub>&phiC31</sub>||0.01 min<sup>-1</sup>||Degradation rate of &phiC31||Assumed | ||
!Total: 15!!!!!!Total: 29.55!! | !Total: 15!!!!!!Total: 29.55!! | ||
|} | |} | ||
{{:Team:ETH Zurich/tpl/foot}} | {{:Team:ETH Zurich/tpl/foot}} |
Revision as of 03:50, 9 October 2014
Parameters
No model is complete without parameters. Our exhaustive list of parameters are summarised in the table below.
Parameter | Value | Description | Reference | |||||
---|---|---|---|---|---|---|---|---|
αLuxR | 60 nMmin-1 | Production rate of LuxR | Estimated | |||||
kRLux | 0.1 nM-1min-1 | Rate of formation of RLux from 30C6HSL and LuxR | Weber | |||||
k-RLux | 10 min-1 | Dissociation rate of RLux | Weber | |||||
kDRLux | 0.05 nM-1min-1 | Dimerization rate of RLux | Weber | |||||
k-DRLux | 1 min-1 | Dissociation rate of DRLux | Weber | |||||
KdPLux | 0.007652 nM | Dissociation rate constant for DRLux-PLux | Fitted | |||||
d30C6HSL | 0.004 min-1 | Degradation rate of 30C6HSL | Estimated/Fitted | |||||
dLuxR | 0.0174 min-1 | Degradation rate of LuxR | Weber | |||||
dRLux | 0.0174 min-1 | Degradation rate of RLux | Weber | |||||
dDRLux | 0.0231 min-1 | Degradation rate of DRLux | Weber | |||||
dBxb1 | 0.01 min-1 | Degradation rate of Bxb1 | Assumed | |||||
αLasR | 60 nMmin-1 | Production rate of LasR | Estimated | |||||
kRLas | 0.1 nM-1min-1 | Rate of formation of RLas from 30C6HSL and LasR | Weber | |||||
k-RLas | 10 min-1 | Dissociation rate of RLas | Weber | |||||
kDRLas | 0.05 nM-1min-1 | Dimerization rate of RLas | Weber | |||||
k-DRLas | 1 min-1 | Dissociation rate of DRLas | Weber | |||||
KdPLas | 1.3672 nM | Dissociation rate constant for DRLas-PLas | Fitted | |||||
d30C6HSL | 0.004 min-1 | Degradation rate of 30C6HSL | Estimated/Fitted | |||||
dLasR | 0.0174 min-1 | Degradation rate of LasR | Weber | |||||
dRLas | 0.0174 min-1 | Degradation rate of RLas | Weber | |||||
dDRLas | 0.0231 min-1 | Degradation rate of DRLas | Weber | |||||
d&phiC31 | 0.01 min-1 | Degradation rate of &phiC31 | Assumed | Total: 15 | Total: 29.55 |