Team:ETH Zurich/modeling/int

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=== Assumptions ===
=== Assumptions ===
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;'''Assumption A'''
 
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:The back-reaction from DBxb1 binding to site inactive is considered to be negligible, compared to the flipping rate. That is to say that once a site is active, it can only be flipped. Thus, an active site would only be a transitional state in our whole cell model. As the flipping is not modeled in our integrase subsystem, active sites are not transformed into flipped sites at the end of the information pipeline. Thus, we consider that we can express the switching rate given active site concentration.
 
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;'''Assumption B'''
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We keep the following assumptions from the previous characterization: '''assumption A''', '''assumption B''' and '''assumption C'''. A assumption is made:
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:As switching needs two active sites to be effective (for more information on flipping, check the [http://2014.igem.org/Team:ETH_Zurich/modeling/xor XOR gate page]), the switching rate is approximated to: $${(\frac{SA_{Bxb1}}{S_{TOT}})}^2$$ This approximation is understated by statistical considerations.
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;'''Assumption C'''
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:Given the normalization of the paper, basal rate of production of Bxb1 is not taken into account. Thus, we consider that $$A_L = 0$$
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;'''Assumption E'''
;'''Assumption E'''

Revision as of 01:02, 18 October 2014

iGEM ETH Zurich 2014