Team:ETH Zurich/modeling/int

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The parameter fitting is based on Bonnet's paper<sup>[[Team:ETH_Zurich/project/references|[9]]]</sup>. Their experimental setup is different from ours. However, they experimentally retrieve a transfer function between aTc and Bxb1 switching rate. Here is the figure of interest in our case.
The parameter fitting is based on Bonnet's paper<sup>[[Team:ETH_Zurich/project/references|[9]]]</sup>. Their experimental setup is different from ours. However, they experimentally retrieve a transfer function between aTc and Bxb1 switching rate. Here is the figure of interest in our case.
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[[File:ETH Zurich Bonnet S4.jpg|center|400px|thumb|Transfer function from aTc to Bxb1. The experimental data corresponds to the points. They fitted this data with their own model. Supplementary figure S4 of Bonnet's paper ''Amplifying Genetic Logic Gates''<sup>[[Team:ETH_Zurich/project/references|[9]]]</sup>]]
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[[File:ETH Zurich Bonnet S4.jpg|center|400px|thumb|Transfer function from aTc to Bxb1. The experimental data corresponds to the points. They fitted this data with their own model. Supplementary figure S4 of Bonnet's paper ''Amplifying Genetic Logic Gates''<sup>[[Team:ETH_Zurich/project/references|[9]]]</sup>].]
To use their experimental data, we have to model their induction mechanism using aTc. We use a simplified version of this phenomenon proposed by the [http://2013.igem.org/Team:UCSF 2013 iGEM team UCSF]. They modeled the induction with aTc as a leaky Hill function. In the following table are the set of parameters introduced to model induction.
To use their experimental data, we have to model their induction mechanism using aTc. We use a simplified version of this phenomenon proposed by the [http://2013.igem.org/Team:UCSF 2013 iGEM team UCSF]. They modeled the induction with aTc as a leaky Hill function. In the following table are the set of parameters introduced to model induction.

Revision as of 22:21, 17 October 2014

iGEM ETH Zurich 2014