Team:ETH Zurich/modeling/int

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(Difference between revisions)
(Characterization: KDBxb1)
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;'''Assumption B'''
;'''Assumption B'''
:As switching needs two active sites to be effective (for more information on flipping, check the [https://2014.igem.org/Team:ETH_Zurich/modeling/xor XOR gate page]), the switching rate is approximated to: $${(\frac{SA_{Bxb1}}{S_{TOT}})}^2$$ This approximation is understated by statistical considerations.
:As switching needs two active sites to be effective (for more information on flipping, check the [https://2014.igem.org/Team:ETH_Zurich/modeling/xor XOR gate page]), the switching rate is approximated to: $${(\frac{SA_{Bxb1}}{S_{TOT}})}^2$$ This approximation is understated by statistical considerations.
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;'''Assumption C'''
 
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:We assume quasi-steady state for SA<sub>Bxb1</sub> and for DBxb1, as they are both involved in binding reactions.
 
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$$\begin{align*}
 
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\frac{d[DBxb1]}{dt} &= k_{DBxb1}*[Bxb1]^2 - k_{-DBxb1}*[DBxb1] - k_{SABxb1}*[DBxb1]*[SI_{Bxb1}] + k_{-SABxb1}*[SA_{Bxb1}] - d_{DBxb1}*[DBxb1] \approx 0 \\
 
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\frac{d[SA_{Bxb1}]}{dt} &= k_{SABxb1}*[DBxb1]*[SI_{Bxb1}] - k_{-SABxb1}*[SA_{Bxb1}] \approx 0
 
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\end{align*}$$
 
;'''Assumption F'''
;'''Assumption F'''

Revision as of 14:43, 12 October 2014

iGEM ETH Zurich 2014