Team:ETH Zurich/modeling/diffmodel

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(Difference between revisions)
m (Initial conditions determined by bead preparation step)
m (Initial conditions determined by bead preparation step)
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The cells of the first row are induced by 10 μ M AHL just before they are encapsulated in alginate. Then, bead formation takes half-an-hour before these cells are added in the first well. During this half-an-hour AHL can diffuse out. Therefore initial conditions in the first cell completely depend on these previous steps. We have also simulated this bead preparation in order to get these initial conditions.
The cells of the first row are induced by 10 μ M AHL just before they are encapsulated in alginate. Then, bead formation takes half-an-hour before these cells are added in the first well. During this half-an-hour AHL can diffuse out. Therefore initial conditions in the first cell completely depend on these previous steps. We have also simulated this bead preparation in order to get these initial conditions.
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[[File:ETHZ Beads in storage solution.jpg|center|600px|thumb|Beads sotred in calcium chloride, 10mM]]
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[[File:ETHZ Beads in storage solution.jpg|float|600px|thumb|Beads stored in calcium chloride, 10mM]]
The geometry is a simple bead in a bigger compartment filled with calcium chloride. We look at the concentrations at the middle of the bead. Initial concentration of LuxR before bead diffusion starts is a classical steady state a<sub>LuxR</sub>/d<sub>LuxR</sub>
The geometry is a simple bead in a bigger compartment filled with calcium chloride. We look at the concentrations at the middle of the bead. Initial concentration of LuxR before bead diffusion starts is a classical steady state a<sub>LuxR</sub>/d<sub>LuxR</sub>

Revision as of 18:19, 17 October 2014

iGEM ETH Zurich 2014