Team:ETH Zurich/modeling/diffmodel

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(Difference between revisions)
(Estimation of parameters from literature)
(Initial conditions determined by bead preparation step)
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Extracellular AHL diffuses very fast through the membrane, which makes intracellular AHL increase, until both reach the same value and diffusion through the membrane reaches an equilibrium. From this point on AHL starts to bind to LuxR to form RLux, so AHL decreases, until RLux triggers enough production of LuxI which catalyses again AHL production, and makes AHL concentration become stable.
Extracellular AHL diffuses very fast through the membrane, which makes intracellular AHL increase, until both reach the same value and diffusion through the membrane reaches an equilibrium. From this point on AHL starts to bind to LuxR to form RLux, so AHL decreases, until RLux triggers enough production of LuxI which catalyses again AHL production, and makes AHL concentration become stable.
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After 30 minutes, almost all LuxR is bound to AHL and converted to RLux, so that we have  
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After 30 minutes, all LuxR is bound to AHL and converted to RLux, so that we have  
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$$ [LuxR]_i= 10nM$$
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$$ [LuxR]_i= 0nM$$
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$$ [RLux]_i= 160 nM$$
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$$ [RLux]_i= 200 nM$$
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$$ [AHLint]_i=[AHLint]_i=2 μ M$$
RLux has already started to trigger the production of LuxI and GFP, so that we have  
RLux has already started to trigger the production of LuxI and GFP, so that we have  
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$$ [mRNAGFP]_i =  24nM$$
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$$ [mRNAGFP]_i =  40nM$$
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$$ [GFP]_i =  180nM$$
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$$ [GFP]_i =  900nM$$
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$$ [mRNALuxI]_i = 24nM$$
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$$ [mRNALuxI]_i = 40nM$$
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$$ [LuxI]_i = 3300nM$$
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$$ [LuxI]_i = 16000nM$$
<html><div id='Estimation'></html>
<html><div id='Estimation'></html>
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=== Estimation of parameters from literature ===
=== Estimation of parameters from literature ===

Revision as of 04:37, 17 October 2014

iGEM ETH Zurich 2014