Team:ETH Zurich/modeling/diffmodel

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(Estimation of parameters from literature)
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According to Lars Müller's master thesis<sup>[[Team:ETH_Zurich/project/references|[29]]]</sup>, cell doubling time is 30 minutes, which gives a growth rate of 0.023 min<sup>-1</sup>. Lars Müller also mentions in his master thesis<sup>[[Team:ETH_Zurich/project/references|[29]]]</sup> that the maximum capacity of his 34 pL beads is 3000 cells, which would correspond to  maximum of N<sub>m</sub> = 8. 10<sup>8</sup> cells per bead in our case (10 &mu;L beads).  
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The initial number of beads is 10 million. According to Lars Müller's master thesis<sup>[[Team:ETH_Zurich/project/references|[29]]]</sup>, in picoliter beads, cells doubling time is 30 minutes. Here we are using beads with a volume in the microliter range. Because of bead volume, oxygen and nutrients are much less accessible. Therefore, we multiplied this doubling time by 4. We have a growth rate of 0.006 min<sup>-1</sup> which is still above the growth rate in anaerobic conditions (0.004 min<sup>-1</sup> according to [http://bionumbers.hms.harvard.edu/search.aspx?log=y&task=searchbytrmorg&trm=growth+rate+e+coli&org= Bionumbers]) )
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In the conditions of lack of oxygen and nutrients where our cells are, we consider that they might at maximum double or triple. So we take 3 . 10<sup>7</sup> cells per bead for N<sub>m</sub>.
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Revision as of 03:44, 17 October 2014

iGEM ETH Zurich 2014