Team:ETH Zurich/labblog/20140901meet

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== Week 15 ==  
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== Week 15 : Integrase parameters retrieved from Bonnet's paper, problems with integrase experiments==  
==== Monday 1st September ====
==== Monday 1st September ====
* We finally managed to retrieve integrase parameters from Bonnet et al.'s <sup>[[Team:ETH_Zurich/project/references#refEmergence|[9]]]</sup> paper steady state behavior curve. You can check  <html> <a targetid="intparam"" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#intparam"> the corresponding article </a> </html>  for more details. We can get Hill function parameters this way, but still need dynamic data from our own experiments to retrieve dynamic parameters (i. e. degradation rates).
* We finally managed to retrieve integrase parameters from Bonnet et al.'s <sup>[[Team:ETH_Zurich/project/references#refEmergence|[9]]]</sup> paper steady state behavior curve. You can check  <html> <a targetid="intparam"" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#intparam"> the corresponding article </a> </html>  for more details. We can get Hill function parameters this way, but still need dynamic data from our own experiments to retrieve dynamic parameters (i. e. degradation rates).
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* Integrase don't seem to switch the terminator at the moment, maybe because of a mutation that we found in their sequence.
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* Integrases don't seem to switch the terminator at the moment, maybe because of a mutation that we found in their sequence.
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Latest revision as of 19:04, 11 October 2014