Team:ETH Zurich/labblog/20140829mod

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== Integrase parameters ==  
== Integrase parameters ==  
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As we did not find these parameters on the web, we used the data given by the Bonnet paper "Amplifying genetic logic gates". The figure S4 is particularly interesting, even if it does not give any information on the dynamics of the system. We modeled and wrote the equations corresponding to Bonnet's experiment.  
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As we did not find these parameters on the web, we used the data given by Bonnet et al.'s paper "Amplifying genetic logic gates" <sup>[[Team:ETH_Zurich/project/references#refEmergence|[9]]]</sup> . The figure S4 is particularly interesting, even if it does not give any information on the dynamics of the system. We modeled and wrote the equations corresponding to Bonnet's experiment.  
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By considering reasonable order of magnitude for other parameters present in the lumped parameters, we find that the value of the dissociation constants are both of the order of 10<sup>-4</sup>. This order of magnitude is not surprising at all, as we know that the binding of integrases to DNA is very specific (which is underlined by the low value of the dissociation constant of this reaction) and that integrases are more stable as dimer than as monomer.
By considering reasonable order of magnitude for other parameters present in the lumped parameters, we find that the value of the dissociation constants are both of the order of 10<sup>-4</sup>. This order of magnitude is not surprising at all, as we know that the binding of integrases to DNA is very specific (which is underlined by the low value of the dissociation constant of this reaction) and that integrases are more stable as dimer than as monomer.
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Latest revision as of 00:38, 18 October 2014