Team:ETH Zurich/labblog/20140825meet

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Week 14

Monday, August 25th

We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.

Diffusion experiment with agar. Check <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> for more details.

Diffusion experiment with liquid. Check <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> for more details.

Diffusion experiment with beads. Check <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> for more details.

We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length.

Comsol simulation of diffusion experiments for 1mM and for 1 μM AHL initial concentration in sender wells. It explains why with 1mM all wells start glowing at the same time.

We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves.

Fitting of quorum sensing dynamic parameters. Check the corresponding article for more details.