Team:ETH Zurich/labblog/20140825meet

Week 14

Monday, August 25th

• We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.

<center> Diffusion experiment with liquid. Check </html> <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> for more details. {{:Team:ETH Zurich/Templates/Video| width=460px| id=video4| ratio=143/100| srcOGG=https://static.igem.org/mediawiki/2014/6/68/20140822_diffusion_s24_liquid_culture.ogg |poster=https://static.igem.org/mediawiki/2014/a/a9/ETH_Zurich_Diffusion3.JPG}}

<center> Diffusion experiment with beads. Check </html> <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> for more details. {{:Team:ETH Zurich/Templates/Video| width=460px| id=video4| ratio=143/100| srcOGG=https://static.igem.org/mediawiki/2014/6/64/ETH_Zurich_diffusion_s24_beads.ogg |poster=https://static.igem.org/mediawiki/2014/2/27/ETH_Zurich_Diffusion4.JPG}}

• We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length.

Comsol simulation of diffusion experiments for 1mM and for 1 μM AHL initial concentration in sender wells. It explains why with 1mM all wells start glowing at the same time.

• We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves.

Fitting of quorum sensing dynamic parameters. Check the corresponding article for more details.