Team:ETH Zurich/labblog/20140825meet

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== Week 14 ==  
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== Week 14 : Diffusion experiments started, simulated with Comsol, and fitting of quorum sensing dynamics ==  
==== Monday, August 25th ====
==== Monday, August 25th ====
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* We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.
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* We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths. Check  <html> <a id="insidelink" targetid="diffusionexp" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html>  for more details.
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*We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length. Check <html> <a id="insidelink" targetid="diffcomsol" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffcomsol"> this article </a> </html> for more details.
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Diffusion experiment with agar. Check  <html> <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html>  for more details.
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Diffusion experiment with liquid. Check <html> <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html>  for more details.
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Diffusion experiment with beads. Check  <html> <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html> for more details.
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*We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length.
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[[File:ETH Zurich Comsol3.PNG|center|900px|thumb|Comsol simulation of diffusion experiments for 1mM and for 1 &mu;M AHL initial concentration in sender wells. It explains why with 1mM all wells start glowing at the same time.]]
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* We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves.
* We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves.
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Check <html> <a id="insidelink" targetid="qsdynamics" href="https://2014.igem.org/Team:ETH_Zurich/blog#qsdynamics"> the corresponding article </a> </html> for more details.
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[[File:ETH Zurich Model4.PNG|center|800px|thumb|Fitting of quorum sensing dynamic parameters. Check <html> <a id="insidelink" class="all" href="https://2014.igem.org/Team:ETH_Zurich/blog#qsparameters"> the corresponding article </a> </html> for more details.]]
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Latest revision as of 19:38, 11 October 2014

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