Team:ETH Zurich/labblog/20140825meet

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Revision as of 09:50, 8 September 2014

Week 14

Monday, August 25th

We carried diffusion experiment with different designs : punched PDMS, liquid, beads. For every design we added high concentrations of pure AHL in the sender well, so cells are glowing a lot. However because of this high concentration, diffusion is very high and at a given AHL concentration, receiving cells are glowing at the same time for all 4 channel lengths.

Diffusion experiment with agar. Click here to watch the video. Check the corresponding article for more details.

Diffusion experiment with liquid. Click here to watch the video. Check the corresponding article for more details.

Diffusion experiment with beads. Click here to watch the video. Check the corresponding article for more details.

We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length.

Comsol simulation of diffusion experiments for 1mM and for 1 μM AHL initial concentration in sender wells. It explains why with 1mM all wells start glowing at the same time.

We could fit degradation rates of external, internal AHL and GFP thanks to quorum sensing dynamic curves.

Fitting of quorum sensing dynamic parameters. Check the corresponding article for more details.

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 [[File:ETH_Zurich_Diffusion3.JPG|center|500px|thumb|Diffusion experiment with liquid. Click [https://2014.igem.org/Team:ETH_Zurich/Video2 here] to watch the video. Check  <html> <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html>  for more details.]]
-[[File:ETH_Zurich_Diffusion4.JPG|center|500px|thumb|Diffusion experiment with agar. Click [https://2014.igem.org/Team:ETH_Zurich/Video3 here] to watch the video. Check  <html> <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html>  for more details.]]
+[[File:ETH_Zurich_Diffusion4.JPG|center|500px|thumb|Diffusion experiment with beads. Click [https://2014.igem.org/Team:ETH_Zurich/Video3 here] to watch the video. Check  <html> <a class="all" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#diffusionexp"> the corresponding article </a> </html>  for more details.]]
 *We have a Comsol simulation for these experiments that explains why at this concentration of AHL (1mM), all 4 wells glow at the same time irrespectively of channel length.