Team:ETH Zurich/labblog/20140818mod

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== Quorum sensing parameter fitting ==  
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== Fitting quorum sensing Hill functions parameters ==  
==== Wednesday, August 18th ====
==== Wednesday, August 18th ====
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The experimental design also allowed us to detect eventual cross-talk with other signaling molecules, the one corresponding to the las system and the one corresponding to the rhl system. No cross-talk was observed with the signaling molecule of the rhl system. However, the las system signaling molecule activated the Lux promoter. We chose to model this cross-talk, which corresponds to the induction of a promoter, by an Hill function. This fitting allowed us to verify that the Hill function was adapted to describe a cross-talk phenomena. The derived parameters indicated that the concentration in the las signaling molecule had to be high in order to have an effect on our system (It was two orders of magnitude away from the needed concentration of AHL to activate the system). Nevertheless, once activated, the effects of these crosstalk are not to be neglected.  
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The experimental design also allowed us to detect eventual cross-talk with other signaling molecules, the one corresponding to the las system and the one corresponding to the rhl system. No cross-talk was observed with the signaling molecule of the rhl system. However, the las system signaling molecule activated the Lux promoter. We chose to model this cross-talk, which corresponds to the induction of a promoter, by an Hill function. This fitting allowed us to verify that the Hill function was adapted to describe a cross-talk phenomena. The derived parameters indicated that the concentration in the las signaling molecule had to be high in order to have an effect on our system (It was two orders of magnitude away from the needed concentration of AHL to activate the system). Nevertheless, once activated, the effects of these crosstalk are not to be neglected. Check <html> <a targetid="cross-talk" id="insidelink" href="https://2014.igem.org/Team:ETH_Zurich/blog#cross-talk"> the following article </a> </html> for more information on the experimental setting.
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These results are promising and we look forward to fit the dynamic data of this system.
These results are promising and we look forward to fit the dynamic data of this system.
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Latest revision as of 19:20, 11 October 2014