Team:ETH Zurich/labblog/20140702meet

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== Title of article ==  
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== Miniprep==  
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= Date =
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= Wednesday, July 2nd =
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===Title of one section===
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===Miniprep piG0001 piG0029===
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bla bla bla
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Addition of 200 μL Resuspension solution to culture pellet, vortex, addition of 200 μL Lysis solution <br/>
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After ca. 5 min addition of 350 μL Neutralization solution, centrifuge samples for >10 min at 4000 rpm<br/>
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Preparation of columns: addition of 500 μL Preparation solution, centrifugation for 1 min at 12 000 rcf, discard flow-through<br/>
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Give supernatant of centrifuged samples onto columns, spin for 1 min at 12 000 rcf, discard flow-through<br/>
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Addition of  500 μL Optional Wash Solution, spin for 1 min at 12 000 rcf, discard flow-through<br/>
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Addition of  750 μL Wash Solution, spin for 1 min at 12 000 rcf, discard flow-through<br/>
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Dry columns by centrifuging them  for 2 min at 12 000 rcf, subsequently place columns in new collection tubes<br/>
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Elute DNA with 100 μL Elution solution<br/>
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According to Sigma-Aldrich Plasmid Miniprep Kit protocol<br/>
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Send samples piG0001, piG0015 and piG0029 for sequencing, primers used: oiG0001 for piG0001 and piG0020, oiG0002 for piG0015
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===Title of one section===
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bla bla bla
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Revision as of 09:48, 10 July 2014

Miniprep

Wednesday, July 2nd

Miniprep piG0001 piG0029

Addition of 200 μL Resuspension solution to culture pellet, vortex, addition of 200 μL Lysis solution
After ca. 5 min addition of 350 μL Neutralization solution, centrifuge samples for >10 min at 4000 rpm
Preparation of columns: addition of 500 μL Preparation solution, centrifugation for 1 min at 12 000 rcf, discard flow-through
Give supernatant of centrifuged samples onto columns, spin for 1 min at 12 000 rcf, discard flow-through
Addition of 500 μL Optional Wash Solution, spin for 1 min at 12 000 rcf, discard flow-through
Addition of 750 μL Wash Solution, spin for 1 min at 12 000 rcf, discard flow-through
Dry columns by centrifuging them for 2 min at 12 000 rcf, subsequently place columns in new collection tubes
Elute DNA with 100 μL Elution solution
According to Sigma-Aldrich Plasmid Miniprep Kit protocol

Send samples piG0001, piG0015 and piG0029 for sequencing, primers used: oiG0001 for piG0001 and piG0020, oiG0002 for piG0015

Top